摘要
目的 :分离大鼠肺血管及腹主动脉的内皮细胞 ,建立研究血管紧张素转换酶 (ACE)表达的体外模型。 方法 :分离大鼠肺血管内皮细胞采用活体胶原酶灌注法、腹主动脉内皮细胞采用贴壁法 ;二种方法均用胰酶不完全消化和加入血清或无血清培养相结合的方法得到纯化的内皮细胞。 结果 :肺血管内皮细胞灌注法细胞传代至第 4代后其增生速度趋于稳定 ,内皮细胞纯度达到 95 %以上 ,冻存后细胞复苏率 (0 .816± 0 .0 85 )和贴壁率 (0 .75 8±0 .0 79)均较高、生长良好。腹主动脉内皮细胞贴壁法第 4代传代后细胞增生速度较慢 ,纯度为 78% ,冻存后传代细胞复苏率为 0 .72 4。 结论 :肺血管内皮细胞灌注法分离到的大鼠肺血管内皮细胞 ,纯度高 。
Objectives:Isolation of rat pulmonary vein endothelial cells and abdominal aorta cells for the expression of angiotensin converting enzyme(ACE). Methods: Rat pulmonary vein endothelial cells (RPVEC) were isolated by collagenase and abdominal aorta cells by mounting method. Two kinds of pure cells were obtained by trypsin digestion and culture with serum containing and serum free medium. Results: Stable RPVEC was obtained after four or five subculture, the purity was above 95%. The rates of resuscitation (0.816±0.085) and reattachment ( 0.758 ±0.079)were very high. Conclusions:RPVEC is highly purified, active, and more stable after subculture, it can be used as a model of ACE regulation in vitro .
出处
《医学研究生学报》
CAS
2001年第z1期37-39,共3页
Journal of Medical Postgraduates
