摘要
为探讨生长抑素(SST)Ⅱ型受体激动剂奥曲肽(OCT)和NC-8-12(生长抑素Ⅱ型受体特异激动剂)是否能在体内、体外实验中抑制大肠癌细胞的增殖,采用MTT法对两株大结肠癌细胞株HCT116、LOVO进行不同生长抑素类似物作用下体外细胞增殖状况的研究。并于裸鼠皮下接种大肠癌细胞株HCT116后,皮下给予OCT和NC-8-12治疗,进行体内细胞增值状况的研究。结果发现体外生长实验:10^(-8)mol/L的OCT或NC-8-12作用HCT116、LOVO细胞株24小时后,能明显抑制胰岛素引起的肿瘤细胞增殖作用。治疗HCT116裸鼠移植癌的实验:OCT和 NC-8-12以300μg/(kg·d)的剂量皮下给药共21天的条件下,对肿瘤的生长(以肿瘤的体积和最终瘤重为观察指标)产生显著抑制作用。本文提示生长抑素类似物OCT和NC-8-12在体内、体外实验中均能抑制大肠癌细胞的增殖。
To confirm the analogue of somatostatin OCT and NC-8-12 on the colon tumor cell growth invitro and vivo. Two colon-cancer cell lines HCT116.LOVO of which cell proliferation was measured with MTTwere treated with different analogue of somatostatin in vitro. The nude mice were implanted s. c. with coloncancer cell line HCT116,then were injected s. c. with OCT and NC-8-12. Results showed that cell proliferation ofHCT116 induced by insulin was obviously inhibited by OCT or NC-8-12 at 10^(-8)mol/L 24h later afte...
出处
《山东医药》
CAS
北大核心
2002年第28期6-8,共1页
Shandong Medical Journal
关键词
生长抑素
大肠肿瘤
医学肿瘤学
Somatostatin
Colonic neoplas
Medical oncology
