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利用T-DNA插入法构建棉花功能基因组研究用的突变体库 被引量:2

Construction of Mutant Populations by T-DNA Insertion for Functional Genomic Study in Cotton
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摘要   The use of transfer DNA (T DNA) as a mutagen has been developed for tagging genes in many crops, and results showed that T-DNA insertion is a random event, and that the inserted genes are stable through multiple generations. Through sequencing PCR-amplified fragments adjacent to the inserted elements, we can construct the T-DNA flanking database,which would be useful for cloning the genes tagged by T-DNA.   ……
作者 Jia-he WU
出处 《棉花学报》 CSCD 北大核心 2002年第z1期48-,共1页 Cotton Science
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