摘要
目的:探讨LAMS对胃癌细胞化疗的增敏作用。方法:RT-PCR及免疫组化法测定LAMS作用胃癌细胞前后Bcl-2基因蛋白含量;MTT法分别测定5-Fu、MTX、MMC、EPI、CTX与LAMS联合及单独作用胃癌细胞的ID50以及有效作用时间。结果:LAMS使胃癌细胞Bcl-2 mR-NA及基因蛋白表达下降,使胃癌细胞对5-Fu、MTX、MMC、EPI、CTX的敏感度增加、有效作用时间延长。结论:LAMS可有效地增加胃癌细胞对5-Fu、MTX、MMC、EPI、CTX的敏感性。
Objective:To study the sensitization effect of laminarin sulphate(LAMS) on the chemotherapy for gastric cancer cells. Methods:RT-PCR and Immunohistochemical stainings were used to determine the change of Bcl-2 protein treated with LAMS.MTT assay was used to examine the ID50 and the effective time of gastric cancer cells treated with 5-Fu,MTX,MMC,EPI,CTX or combined with LAMS.Results:The expression of Bcl-2 mRNA and protein decreased in the gastric cancer cells treated with LAMS.The sensitivity of human gast...
出处
《激光杂志》
CAS
CSCD
北大核心
2008年第4期88-,90,共2页
Laser Journal
