摘要
目的:评价巢式PCR和免疫组化技术在检测肾癌组织支原体中的应用和价值。方法:采用支原体通用引物巢式PCR技术和单克隆抗体PD4免疫组化技术,检测95例肾癌组织中支原体DNA和支原体p40蛋白表达。结果:免疫组化检测和巢式PCR检测的阳性检出率分别为64.2%和81.1%,巢式PCR技术检测肾癌组织支原体在灵敏度、特异度、约登指数、阳性预测值和阴性预测值方面均优于免疫组化技术。结论:巢式PCR和免疫组化技术均可作为肾癌组织支原体的检测方法,巢式PCR较免疫组化检测敏感。
Objective:To investigate the comparison and estimation of nested PCR and Immunohistochemistry (IHC) in the detection of mycoplasma in renal cell carcinoma (RCC). Methods: There were 95 samples of archived embedded tissues of RCC. Mycoplasma DNA and mycoplasma p40 in RCC were detected by nested PCR with mycoplasma universal primer and by IHC with mycoplasma monoclonal antibody (MAb) PD4 respectively. Results: The positive detection ratio of IHC and nested PCR were 64.2% and 81.1%, respectively. It was showed...
出处
《陕西医学杂志》
CAS
北大核心
2008年第9期1216-1218,1221,共4页
Shaanxi Medical Journal
基金
陕西省科学技术研究发展计划项目(No2008k09-04)
关键词
肾肿瘤/诊断
支原体感染/诊断
聚合酶链反应
免疫组织化学
对比研究
Kidney neoplasms/diagnosis Mycoplasmas infections/diagnosis Polymerase chain reaction Immunohistochemistry Comparative study
