摘要
研究 4 羟基 2 壬烯酸对体外培养的主动脉内皮细胞作用 ,以探讨动脉粥样硬化的发病机理。采用单细胞凝胶电泳检测DNA损伤 ,对体外培养的主动脉内皮细胞在不同浓度 4 羟基 2 壬烯酸作用下产生的DNA损伤进行检测。结果发现 ,体外培养的主动脉内皮细胞分别用 5 μmol L、10 μmol L和 15 μmol L 4 羟基 2 壬烯酸处理 10h后彗星试验的尾距分别为 32 .8± 1.1、4 4 .3± 1.0和 74 .6± 1.0 ,与未用 4 羟基 2 壬烯酸处理的正常对照组彗星试验的尾距 (6 .0± 0 .7)比较 ,差异有显著性 (P <0 .0 0 1) ,经 1μmol L 4 羟基 2 壬烯酸处理后的主动脉内皮细胞彗星试验的尾距为 11.3± 0 .9,与正常对照组比较 ,两者之间差异无显著性 (P >0 .0 5 )。结果提示 ,4 羟基 2 壬烯酸可导致体外培养的主动脉内皮细胞的DNA损伤 ,并随着 4 羟基 2 壬烯酸的浓度增高DNA损伤加剧。
Aim To study the effects of 4-hydroxy-2-nonenal (HNE) on cultured human aortic endothelial cells so as to explore the mechanism of the pathogenesis of atherosclerosis. Methods In single cell gel electrophoresis, quantitative DNA damage detection was used to identify the DNA damage at different concentrations in cultured human aortic endothelial cells. Results Tail moment was 32.8±1.1, 44.3±1.0 and 74.6±1.0 when human aortic endothelial cells were treated with 5 μmol/L, 10 μmol/L and 15 μmol/L of HNE for 10 hours, which were of statistical significance when compared with the normal group (6.0±0.7, P<0.001 respectively), but when human aortic endothelial cells was treated with 1 μmol/L of HNE, the tail moment was 11.3±0.9, which was of no statistical difference compared with the untreated group(P>0.05). Conclusion HNE induces DNA damage of cultured aortic endothelial cells. The DNA damage levels are proportional to the HNE concentrations.
出处
《中国动脉硬化杂志》
CAS
CSCD
2004年第6期677-679,共3页
Chinese Journal of Arteriosclerosis
