摘要
目的探讨阿魏酸钠(sodium femlate,SF)对过氧化氢(hydrogen peroxide,H_2O_2)引起的PC12细胞凋亡保护作用的可能机制。方法在H_2O_2诱导PC12细胞凋亡模型的基础上,采用MTT比色分析检测细胞存活率,RT-PCR检测前列腺凋亡响应基因Par-4(prostate apoptosis response-4,Par-4)和核转录因子-κB p65(nuclear factor-kappaB,NF-KB)mRNA表达,Westem blot检测Par-4和NF-κBp65蛋白表达。结果不同剂量SF预处理1h可提高PC12细胞的存活率,降低Par-4和NF-κBp65的mRNA表达以及蛋白表达。结论SF可剂量依赖性地对抗H_2O_2的神经素毒性作用,其机制可能与凋亡基因Par-4表达有关,与NF-κB的关系尚需进一步探讨。
Objective To explore the possible mechanism of protection of sodium ferulate (SF) against PC12 cell apoptosis induced by hydrogen peroxide(H_2O_2).Methods Based on PC12 cell apoptosis induced by H_2O_2, cell survival rate was determined by MTr colorimetric analysis,prostate apoptosis response-4(Par-4)mRNA ex- pression and nuclear factor-kappa B(NF-κB)mRNA expression determined by reverse transcription-polymerase chain reaction(RT-PCR),Par-4 and NF-κB protein expression determined by Western blot method.Resu...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2005年第S1期25-29,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
广东省重点学科重点项目资助课题(No.2008)
关键词
阿魏酸钠
过氧化氢
PC12细胞
凋亡
PAR-4
NF-KB
sodium ferulate
hydrogen peroxide
PC12 cells
apoptosis
prostate apoptosis response-4
nuclear factor-kappa B
