摘要
目的观察激活负性共刺激信号PD1-PDL1对延长移植肾存活的效能。方法以PDL1Ig基因重组腺病毒为载体,将PDL1Ig基因转入BN大鼠树突状细胞(DC)细胞同时输注受体,以Lewis大鼠为受体,行同种肾移植为转染组,并以未转染DC输注为对照组;观察移植肾存活时间和术后肾功能变化。结果转染组移植肾存活(41±7.6)d,较对照组移植肾存活时间(8.6±1.2)d明显延长;移植组术后血清肌酐较同期对照组明显为低。结论PDL1基因修饰的DC可以明显延长移植肾存活时间。
Objective To observe the efficacy of the hypothesis that stimulating programmed death 1(PD-1)-triggered negative signals on the survival of renal allografts.Methods The dendritic cells(DCs) of BN rats were transfected with PDL1Ig gene-recombined adenovirus.The transfected kidneys were transplanted to Lewis rats(transfection group).BN→Lewis kidney transplantation with non-transplanted kidneys served as controls.The allograft survival time and the allograft function between the two groups were compared.Results The allograft survival time of transfection group was longer than that of controls significantly((41±7.6)d vs(8.6±1.2)d,P<0.05)).The serum creatinine of transfection group at postoperative 8d was lower than that of controls at postoperative 8d.Conclusion DCstransfected by PDL1Ig gene can prolong the survival time of renal allograft significantly.
出处
《重庆医学》
CAS
CSCD
2006年第16期1450-1451,共2页
Chongqing medicine
基金
国家自然科学基金资助项目(30571863)
