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草坪镰刀枯萎病菌拮抗木霉菌的筛选及拮抗机制的研究 被引量:6

Screening of Antagonistic Trichoderma Isolates Against Turfgrass Fusarium oxysporum and Their Antagonistic Mechanism
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摘要 通过木霉属5个菌株与大连高尔夫球场上草坪镰刀枯萎病菌的对峙培养试验,结果表明:哈茨木霉1、哈茨木霉2、钩状木霉及桔绿木霉的抑菌效果很好,可以作为草坪镰刀枯萎病菌的生物防治拮抗菌加以利用,在这5株木霉中以哈茨木霉2的拮抗作用最强。观察结果表明,木霉对镰刀枯萎病菌的拮抗机制主要表现为生长竞争、重寄生及产生抗菌物质使病菌菌丝消解,而木霉菌生长迅速,可附着或缠绕于病菌菌丝上,可产生吸器侵入菌丝。另外,木霉菌还能分泌使病原菌菌丝原生质浓缩、断裂、消解的物质。 The antagonistic experiment between Trichoderma spp.and Fusarium oxysporum,the turfgrass fusarium blight pathogen occurring in Dalian golf courses,showed that T.harzianum 1,T.harzianum 2,T.hamatum,T.citrinoviride exhibited evident inhibiting effect against the soil-borne pathogens.They are of potential biocontrol agents of the Fusarium oxysporum.Among the tested Trichoderma isolates,T.harzianum 2 showed the strongest antagonistic effect.The examination of antagonistic mechanism showed that Trichoderma spp.hyphae growed quickly,the hyphae could adhere to coil around the hyphae of the pathogen.Trichoderma spp.could also secret antibiotic substances to make the pathogen hyphal protoplasm break,condense and rupture.
出处 《中国草地学报》 CSCD 2006年第6期56-60,共5页 Chinese Journal of Grassland
关键词 木霉菌 草坪 草坪镰刀枯萎病菌 拮抗作用 Trichoderma Fusarium oxysporum Turfgrass Antagonistic effect
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  • 1[21]Backman PA,Rodriguez Kabana R.A system for the growth and delivery of biological control agents to the soil[J].Phytopathology,1975,65:819-821. 被引量:1
  • 2[22]Bruce U,Srinivasan Syaines H J.Chitinase and laminarinase production in liquid culture by Trihhoderma spp.and their role in biocontrol of wood decay fungi[J].International Biodeterioration and Biodegradation,1995,35(4):337-353. 被引量:1

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