摘要
目的:观察慢性丙型肝炎患者干扰素治疗前后白细胞介素-18(IL-18)、白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)水平的变化,探讨它们在慢性丙型肝炎发病中的作用。方法:酶联免疫吸附(ELISA)法检测15例正常人、30例丙型肝炎病毒携带者及30例慢性丙型肝炎患者(包括干扰素治疗前、后)血清中细胞因子水平。结果:慢性丙型肝炎患者血清中上述细胞因子显著高于正常对照组(P<0.05)及丙型肝炎病毒携带者组(P<0.05),其值与谷丙转氨酶(GPT)呈显著正相关;经干扰素治疗后上述细胞因子水平均明显降低(P<0.05),IFN抗病毒治疗前血清中IL-10水平由高到低依次为无应答组>部分应答组>完全应答组。结论:上述细胞因子共同参与慢性丙型肝炎的发病过程,并可用于评价干扰素对于机体免疫状态的影响;其中血清中IL-10水平对预测抗病毒疗效有重要意义。
Objective:To observe the changes of serum levels of IL-18,IL-10 and TNF-αin the patients with chronic hepatitis C before and after IFN treatment and to discuss the roles of the above- mentioned serum cytokines in the pathogenisis of chronic hepatitis C.Methods:To detect the levels of IL-18,IL-10 and TNF-α in 15 healthy controls, 30 asymptomatic HCV carriers and 30 patients with chronic hepatitis C ( before and after IFN treatment )by enzyme linked immunosorbent assay (ELISA).Results:The levels of IL-18,IL-10 and TNF-α in the patients with chronic hepatitis C were higher than those in healthy controls(P<0.05) and in asymptomatic HCV carriers(P<0.05).The valus of the above-mentioned cytokines showed a significant positive correlation to GPT.The levels of above-mentioned cytokines decreased obviously after IFN treatment(P<0.05),while the serum levels of IL-10 reduced in sequence in no-response group,partial-response group and complete-response group.Conclusion:IL-18,IL-10 and TNF-αco-participate in the pathogenisis of chronic hepatitis C,and are probably used to evaluate the effect of IFN on the immunical state of patients,and IL-10 is important for predicting the anti-viral efficacy of IFN.
出处
《现代医药卫生》
2006年第19期2919-2921,共3页
Journal of Modern Medicine & Health
