摘要
目的建立高效液相色谱法测定多潘立酮片的含量及含量均匀度。方法采用C18(200mm×4.6mm,5μm)色谱柱,甲醇-0.05mol.L-1磷酸二氢钾溶液(55∶45)(用磷酸调节pH值为4.0±0.05)为流动相,检测波长为286nm,流速为0.8mL.m in-1。结果多潘立酮在0.19264μg^2.50432μg范围内与峰面积呈良好的线性关系(r=1.0000,n=7),平均回收率为100.26%(n=7),RSD为0.49%。结论本方法快速、简便,结果准确,可用于多潘立酮片的质量控制。
OBJECTIVE To establish a HPLC method to determine Domperidone tablets and its content uniformity.METHODS A HPLC assay was developed.Chromatographic assay was performed on a column of Hypersil C_(18)(200mm×4.6 mm,5μm),using methanol-0.05mol· L^(-1) KH_(2)PO_(4)(55∶45)(adjusting to pH 4.0±0.05 by H_(3)PO_(4))as the mobile phase with the flow rate of 0.8mL·min^(-1).The detective wavelength was at 286nm.20μL sampler and external method were used. RESULTS A Satisfactory separation was obtained.The calibration cu...
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2006年第S1期641-642,共2页
Chinese Journal of Modern Applied Pharmacy
