摘要
目的探讨全反式维甲酸(ATRA)诱导雄激素非依赖型前列腺癌细胞系DU145凋亡过程中半胱氨酰天冬氨酸特异性蛋白酶(caspase)3及其细胞骨架蛋白F-actin的变化。方法应用吖碇橙(AO)染色,荧光显微镜观察细胞凋亡形态,流式细胞术(FACScan)测定ATRA对DU145细胞凋亡峰的形成,应用Westernblot测定ATRA在DU145细胞凋亡过程中caspase-3表达的变化,免疫荧光染色观察F-actin纤丝的形态。结果荧光显微镜观察细胞凋亡数目增多,48h时凋亡细胞比例为85.0%,流式细胞术检测可观察到凋亡峰,Westernblot检测显示裂解的caspase-3表达增多,免疫荧光染色可见F-actin的正常结构被破坏。结论ATRA诱导DU145细胞凋亡可能通过caspase-3介导,并导致细胞骨架蛋白破坏。
Objective To investigate the change of caspase-3 and F-actin in the apoptosis of prostate cancer androgen-independent cell line DU145 induced by all-trans retinoic acid (ATRA). Methods Using AO dying and fluorescent microscope to observe the shape of the cell,and the apoptosis peak can be observed by FACScan too.The effects of ATRA on the expression of caspase-3 was analyzed by Western blot and the F-actin’s structure was observed by immunofluorescent microscope. Results Flurescent microscope can observe mo...
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2006年第S1期70-72,共3页
Chinese Journal of Urology
