摘要
通过分枝杆菌(Mycobacteriumsp.)M3限制性降解胆固醇侧链获得了产物雄甾-4-烯-3,17-二酮(AD)和雄甾-1,4-二烯-3,17-二酮(ADD)。优化了胆固醇的投料时间、投料方式、培养基初始pH和葡萄糖浓度等工艺参数。将羟丙基-β-环糊精(HP-β-CD)应用于转化反应中,确定了HP-β-CD的最佳添加时间和添加量,使AD(D)生成率由初始对照的30%提高到60%,转化至72 h时AD(D)生成率达48%,是同期对照的4.0倍,生成率与生成速率均得到显著提高。在添加HP-β-CD的最佳转化条件下,AD(D)生成率达到70%,是初始对照的2.3倍。
Cholesterol can be cleavaged by Mycobacterium sp.M3 to androst-4-ene-3,17-dione AD(D) and androsta-,4-diene-3,17-dione(ADD).Fermentation parameters such as substrate addition time,initial pH,and concentration of glucose in the fermentation culture were optimized.The conversion rate of AD(D) had been raised to 60% from the original 30% in the presence of HP-β-CD,and it was 4 times to the contrast when the conversion time was 72 h,the conversion rate and speed of AD(D) had been obviously raised.In the optimiz...
出处
《生物加工过程》
CAS
CSCD
2008年第2期22-27,共6页
Chinese Journal of Bioprocess Engineering
基金
国家自然科学基金资助项目(200776111)
天津市应用基础研究重点资助项目(043802511)
