摘要
目的通过观察高胆固醇血症患者血管紧张素Ⅱ与其1型受体表达的变化,探讨肾素—血管紧张素系统在动脉粥样硬化发生中的作用。方法在本院健康查体中心随机选取健康对照40例(对照组)和高胆固醇血症患者60例(高脂组),分别自肘静脉取血,分离血清、血浆并提取血小板。放射免疫法检测血浆的血管紧张素Ⅱ水平,逆转录聚合酶链反应和Western blot方法分别检测血小板1型受体表达的mRNA和蛋白质表达水平。结果高脂组的血浆血管紧张素Ⅱ水平较对照组明显升高(92.13±25.27比50.85±21.12,P<0.01),血小板的1型受体mRNA和蛋白质表达较对照组明显升高(0.93±0.22比0.25±0.06,P<0.01;1.35±0.32比0.42±0.10,P<0.01);高脂组血管紧张素Ⅱ与1型受体表达的相关分析显示,1型受体表达与血浆的血管紧张素Ⅱ呈显著正相关(r=0.369,P<0.01)。结论高胆固醇血症患者血小板1型受体的表达增高与血浆血管紧张素Ⅱ浓度增加有关。
Aim To investigate the effects of renin angiotensin system (RAS) in atherosclerosis by observing the change of angiotensinⅡ type 1 (AT1) receptor expression and the effects of statin on it. Methods Sixty patients with hypercholesterolemia and forty healthy persons were investigated as hyperlipidemia group (Hyperlipidemias) and control group (Controls) respectively. Blood samples of ulnar vein were extracted from Controls and Hyperlipidemias. Blood serum, plasma and platelet were extracted and stored at a refrigerator of -80℃. The level of plasma angiotensinⅡ was detected by the method of radioimmunity. While the expression of AT1 mRNA and protein on platelet were assessed by reverse transcription polymerse chain reaction (RT-PCR) and Western blot respectively. Results The plasma angiotensinⅡ of Hyperlipidemias was higher than that of Controls (92.13±25.27 vs 50.85±21.12, P<0.01). The expression of AT1 mRNA and protein on platelets were higher in Hyperlipidemia than those of Controls(0.93±0.22 vs 0.25±0.06, P<0.01 and 1.35±0.32 vs 0.42±0.10, P<0.01). The expression of AT1 protein was directly correlated with the plasma angiotensinⅡ (r=0.369, P<0.01). Conclusions The increase of AT1 mRNA and protein on platelets is related with plasma angiotensin Ⅱ in patients with hypercholesterolemia.
出处
《中国动脉硬化杂志》
CAS
CSCD
2007年第3期169-172,共4页
Chinese Journal of Arteriosclerosis
基金
山东省医疗卫生系统杰出学科带头人(1020工程)项目
山东省科技研究项目(2000BB1CJA3)
山东省自然科学基金(Y2003C04
2003HW069)
青岛市科学技术局资助(02-2-kj-yn-25)
