摘要
目的通过观察银杏叶提取物注射液(金纳多)对脂多糖(LPS)导致的急性肺损伤大鼠炎症介质释放的影响,从而探讨金纳多对急性肺损伤的保护作用。方法63只大鼠随机分成对照组(尾静脉注射生理盐水)、肺损伤组(尾静脉注射LPS,5mg/kg),治疗组(尾静脉注射LPS5mg/kg+金纳多17.5mg/kg),每组21只。注射后2、6、10h各组分别取7只大鼠获得肺泡灌洗液和血液标本以及肺组织切片,进行检测。结果损伤组中肺系数(0.345±0.031)、肺泡灌洗液总蛋白含量(242±3.97)ng/ml,肺通透指数(2.86±0.85)以及血液中肿瘤坏死因子-α[TNF-α,(2.48±0.12)]g/ml、白介素-6[IL-6,(0.546±0.099)ng/ml]、一氧化氮([NO,(72.9±5.3)mmol/L]均较对照组0.252±0.027,(65.04±2.57)ng/ml,0.75±0.13,(0.88±0.04)ng/ml,(0.078±0.004)ng/ml,(21.8±1.5)mmol/L明显上升(均P<0.05),肺组织病理切片显示肺泡结构破坏严重,而应用金纳多后治疗组各项指标为0.271±0.03、(143.19±13.04)ng/ml,1.67±0.97,(1.64±0.24)ng/ml,(0.18±0.055)ng/ml,(39.7±5.6)mmol/L有明显下降(P<0.05),并且肺泡结构破坏程度减轻。结论应用金纳多LPS导致的实验性肺损伤大鼠的炎症介质释放过程有抑制作用,肺泡破坏减轻,提示银杏叶提取物注射液可以减轻大鼠实验性肺损伤的程度,可以保护肺组织。
Objective To investigate the protective effect of injection of ginkgo biloba leaves extract on acute lung injury in rats Methods Sixty three Wistar rats were randomized into three groups(n=21 in each group): group C (control group) in which rats were injected with normal saline into vena caudalis, group I (lung injury group),in which rats were injected with lipopolysaccharide (LPS, 5mg/kg) and group T (treatment group),in which rats were injected with LPS 5mg/kg and extract of ginkgo biloba 17.5mg/kg into vena caudalis). After 2h, 6h, 10h of injection the blood samples and bronchoalveolar lavage fluid were collected, and pathological sections from lung in 7 rats from every group were examined. Results In group I, the levels of lung coefficient 0.345±0.031, the protein concentration of BALF(242±3.97)ng/ml, the lung penetrating index(LPI)(2.86±0.85)and the TNF-α(2.48±0.12)ng/ml, IL-6(0.546±0.099)ng/ml, NO(72.9±5.3)mmol/L levels in blood samples were all higher than those in group C(0.252±0.027,65.04±2.57)ng/ml,(0.75±0.13,0.88±0.04)ng/ml,(0.078±0.004)ng/ml,(21.8±1.5)mmol/L, respectively. In group I, the degree of disorganization of lung alveolus was very serve, and in group T the corresponding parameters 0.271±0.03,(143.19±13.04)ng/ml,1.67±0.97,(1.64±0.24)ng/ml,(0.18±0.055)ng/ml,(39.7±5.6)mmol/L, respectively were significantly lower. Conclusion Ginkgo biloba extract can suppress the mediators of inflammation of the ALI induced by lipopolysaccharide, and it can alleviate disorganization of lung alveolus.
出处
《浙江医学》
CAS
2007年第2期130-132,193,共4页
Zhejiang Medical Journal
关键词
银杏液提取物注射液
急性肺损伤
脂多糖
大鼠
炎症介质
Extract of ginkgo biloba leaves injection Acute lung injury Lipopolysaccharide Rat Mediators of inflammation
