摘要
目的:节约实验经费并解决阳性标本来源困难的问题。方法:用硫酸铝钾液或枯矾液代替待检血清,隔水煮死的变形杆菌液代替伤寒O(TO)菌液,甲醛处理的变形杆菌液代替伤寒H(TH)菌液,隔水煮死的白色葡萄球菌(白葡菌)液代替甲型副伤寒H(PA)和乙型副伤寒H(TB)菌液,模拟肥达氏反应做试管凝集试验。结果:加煮死的变形杆菌液的试管出现梯度颗粒状凝集,加甲醛处理的变形杆菌液的试管出现梯度絮状凝集,加白葡菌液的试管出现不凝集的小圆点。结论:采用本法可做为肥达氏反应的替代方法,既简便又经济有效地开出该项教学试验。
Objective To save the cost of experiment and to solve the problem about the difficulties of getting positivespecimens. Methods To replace blood serum with Potassium aluminum sulfate or the litter alum,to replace bacteria liquid of typhoid O(TO) bacilli with bacteria liquid of bacillus proteus which died of being boiled,replace bacteria liquid of typhoid H (TH)bacilli with bacteria liquid of bacillus proteus treated by solution of formaldehyde,replace H bacteria liquid of Salmonella paratyphi A(PA) and H bac...
出处
《实用医技杂志》
2007年第17期2380-2381,共2页
Journal of Practical Medical Techniques
关键词
肥达氏反应
凝集
替代
Widals reaction
Agglutination
Alternative
