摘要
本工作研究放射免疫检测用的聚苯乙烯球珠辐射接枝丙烯酰胺的情况,並观察经化学处理的球珠(改性球珠)结合免疫球蛋白的能力。实验结果表明,接枝反应在3h内基本完成,接枝温度60℃左右接枝率最大,高剂量率辐照接枝率较低,吸收剂量和单位浓度增加,接枝率也增加,辐照时生成的过氧化物在室温下的分解半衰期约50h。改性球珠结合免疫球蛋白的能力大于原球珠,並随接校率的增加呈现有规律的变化。
In this work radiation grafting of acrylamide onto polystyrene beads by peroxide has been studied and the ability for binding immunoglobulin onto chemically-treated grafted beads has been determined. The results show that the grafting reaction basically completes in three hours.At about 60℃ of grafting reaction temperature the grafting level is the highest. The grafing level is higher in low dose rate than in high dose rate when the total dose is the same.The grafting level is increased with the increasing ...
出处
《同位素》
CAS
北大核心
1990年第3期170-174,160,共6页
Journal of Isotopes
