摘要
目的:研究基质金属蛋白酶(matrix metalloproteinases,MMPs)在翼状胬肉中的分布和活性状态,探讨MMPs在翼状胬肉病理过程中的作用。方法:翼状胬肉切除术患者26例27只眼,静止期18只眼,进行期9只眼,正常人球结膜6例,免疫组织化学法和明胶酶谱法分析MMP-2和MMP-9的表达和活性水平。结果:翼状胬肉标本有MMP-2和MMP-9表达,MMB-2主要分布在基底上皮细胞层,MMP-9在炎症细胞和血管内皮细胞有阳性染色,翼状胬肉静止期MMP-2的活性水平[(235±18.17)扫描单位]和进展期[(221±22.53)扫描单位]比较差异无显著意义(P>0.05),MMP-9活性水平在静止期[(46±32.21)扫描单位]和进展期[(159±19.28)扫描单位]比较差异有显著性意义(P<0.05)。结论:翼状胬肉中MMP-2和MMP-9表达增强,MMP-2和MMP-9活性水平升高可能与翼状胬肉的发展有关。
Purpose: To determine the cellular distribution and activation of MMP-2 and MMP-9 in human pterygium.Methods: The expression and activation of MMP-2 and MMP-9 were determined by immunohistochemistry and quantitative gelatinase zymography from 27 pterygia (26 cases, 18 eyes in stationary pterygia and 9 eyes in progressive pterygia) and 6 normal conjunctival specimens.Results: MMP-2 was expressed in all pterygium examined, specifically localized to the epithelium.MMP-9 was the most abundant in pterygium vascu...
出处
《眼科学报》
2004年第4期242-245,共4页
Eye Science
关键词
翼状胬肉
结膜
基质金属蛋白酶
发病机制
pterygium
conjunctiva
matrix metalloproteinases
pathogenesis
