摘要
目的观察外源性非酶糖基化终产物(AGEs)诱导的大鼠模型血管内皮生长因子(VEGF)在视网膜细胞中的定位。方法应用鼠血清白蛋白(RSA)体外孵育AGEs修饰蛋白,并将其注入健康大鼠体内,每日一次,连续两周。对大鼠视网膜行免疫荧光双重标记,激光共聚焦显微镜下观察胶质纤维素酸性蛋白(GFAP)与VEGF的共表达。结果C组及R组大鼠视网膜内界膜下、神经节细胞层见GFAP阳性染色,但整个视网膜未见VEGF阳性染色。A组大鼠除视网膜内界膜下、神经节细胞层有GFAP阳性染色外,其余各层也可见丝条状贯穿于内外界膜之间的GFAP阳性染色,并且在上述部位有GFAP和VEGF的共表达。结论AGEs可使大鼠视网膜Müller细胞分泌VEGF。
Objective To investigate the immunocytochemical location of advanced glycated end products(AGEs) on the expression of vascular endothelial growth factor(VEGF) in the rats.Methods AGEs were prepared by incubating rat serum albumin(RSA) with D-glucose,then infused it to healthy rats.The immunohistochemical with double fluorescent labeling was performed.The co-expression of glial fibrillary acidic protein(GFAP) and VEGF was oberserved by using a confocal laser scanning microscope.Results The GFAP staining located in the inner limiting membrane and ganglion cell layer among C and R groups.The VEGF staining wasn't obeserved in the entire retina.The GFAP staining was present between inner limiting membrane and outer limiting membrane besides ganglion cell layer,VEGF and GFAP were co-expressed in the same cell among A group.Conclusion Advanced glycation end products can induced the secretion of VEGF among Müller cells.
出处
《中国实验诊断学》
2007年第11期1501-1503,共3页
Chinese Journal of Laboratory Diagnosis
关键词
糖基化终末产物
糖尿病视网膜病变
血管内皮生长因子
Advanced non-enzymatic glycation end products
Diabetic retinopathy
Vascular endothelial growth factor
