摘要
目的探讨胆道闭锁(biliary atresia,BA)患儿肝脏中血小板源性生长因子-BB(platelet-derived growth factor,PDGF-BB)、锌指转录因子(Snail 1)和上皮间质转化(epithelial-mesenchymal transition,EMT)的表达情况以及三者之间的关系。方法回顾性分析2018年1月至2019年12月河北医科大学第二医院小儿外科收治的BA患儿25例(BA组)、胆总管囊肿患儿15例(CCC组)以及肝脏肿瘤患儿10例(NL组)的临床资料;收集患儿活检组织,分别应用免疫组织化学法、实时荧光定量PCR检测PDGF-BB、Snail 1、E钙黏素(E-cadherin)和N钙黏素(N-cadherin)的表达情况,并对上述指标进行相关性分析。结果BA组患儿肝脏中PDGF-BB、Snail 1和N-cadherin的mRNA和蛋白表达均显著高于CCC组和NL组,差异有统计学意义(P<0.05),E-cadherin的mRNA和蛋白表达均显著低于CCC组和NL组,差异有统计学意义(P<0.05)。其中PDGF-BB与Snail 1 mRNA和蛋白的表达呈正相关(r=0.772,P<0.05;r=0.780,P<0.05);PDGF-BB与N-cadherin mRNA和蛋白表达呈正相关性(r=0.835,P<0.05;r=0.702,P<0.05);PDGF-BB与E-cadherin mRNA和蛋白表达呈负相关(r=-0.756,P<0.05;r=-0.696,P<0.05);Snail 1与N-cadherin mRNA和蛋白表达呈正相关(r=0.723,P<0.05;r=0.702,P<0.05);Snail 1与E-cadherin mRNA和蛋白表达呈负相关(r=-0.720,P<0.05;r=-0.636,P<0.05)。肝纤维化程度与PDGF-BB的表达呈正相关(r=0.977,P<0.05);肝纤维化程度与E-cadherin蛋白表达呈负相关(r=-0.722,P<0.05);肝纤维化程度与N-cadherin蛋白表达呈正相关(r=0.711,P<0.05);肝纤维化程度与Snail 1表达呈正相关(r=-0.810,P<0.05)。结论PDGF-BB可能通过增强转录因子Snail 1的转录活性,促进BA患儿发生EMT,这为BA患儿的诊治提供了新的理论依据。
Objective To explore the hepatic expression of platelet-derived growth factor-BB(PDGF-BB),Snail 1 and epithelial-mesenchymal transition(EMT)in children of biliary atresia(BA)and examine the relationship among three parameters.Methods From January 2018 to December 2019,there were BA(n=25),choledochal cysts(CCC,n=15)and liver tumors(NL,n=10).After collecting biopsy tissues,immunohistochemistry and real-time fluorescent quantitative polymerase chain reaction(PCR)were employed for detecting the expression of PDGF-BB,Snail 1,E-cadherin and N-cadherin and analyzing their correlations.Results mRNA and protein expressions of PDGF-BB,Snail 1 and N-cadherin were significantly higher in liver of BA group than those of CCC/NL group(P<0.05);mRNA and protein expressions of E-cadherin were significantly lower than that of CCC/NL group(P<0.05).PDGF-BB was correlated positively with Snail 1 mRNA and protein expressions(r=0.772,P<0.05;r=0.780,P<0.05);PDGF-BB was correlated positively with N-cadherin mRNA and protein expressions(r=0.835,P<0.05;r=0.702,P<0.05);PDGF-BB and E-cadherin mRNA and protein expressions were correlated negatively(r=-0.756,P<0.05;r=-0.696,P<0.05);Snail 1 and N-cadherin mRNA and protein expression were correlated positively(r=0.723,P<0.05;r=0.702,P<0.05);Snail 1 was correlated negatively with E-cadherin mRNA and protein expressions(r=-0.720,P<0.05;r=-0.636,P<0.05).Conclusion In BA children,PDGF-BB may promote EMT through enhancing the transcription activity of transcription factor Snail 1.Thus it may provide new theoretical rationales for diagnosing and treating BA children.
作者
周辉
李索林
时保军
仲智勇
方燕斌
杨晓锋
Zhou Hui;Li Suolin;Shi Baojun;Zhong Zhiyong;Fang Yanbin;Yang Xiaofeng(Department of Pediatric Surgery,Second Hospital,Hebei Medical University,Shijiazhuang 050000,China)
出处
《临床小儿外科杂志》
CAS
CSCD
2023年第3期238-243,共6页
Journal of Clinical Pediatric Surgery
基金
河北省政府资助临床医学优秀人才培养计划项目(2019008)
关键词
胆道闭锁
血小板源性生长因子
上皮-间质转化
蛋白质生物合成
Biliary Atresia
Platelet-Derived Growth Factor
Epithelial-Mesenchymal Transition
Protein Biosynthesis
