摘要
目的:建立高效液相色谱测定葛根芩连丸中葛根素含量的方法。方法色谱柱:Diamonsil C18(250x4.6 mm,5μm);流动相:甲醇-水(15:85);流速:1 mL/min;波长:250 nm;柱温:30℃。结果葛根素浓度在2.0~50.0μg/mL(r=0.9999)范围内线性关系良好,平均回收率为99.8%(RSD=1.03%)。结论建立的方法简便、准确可靠,可用于葛根芩连丸中葛根素含量测定。
Objective To establish a HPLC method for determination of puerarin in Gegen Qinlian pellets.Method Chromatographic column:Diamonsil?C18(250x4.6 mm,5μm);mobile phase:methanol-water(15:85);flow rate:1.0 mL·min-1;detection wavelength:250 nm;column temperature:30℃.Result Puerarin showed a good linear relationship at the range of 2.0~50.0μg·mL-1(r=0.9999).The average recovery of puerarin was 99.8%(RSD=1.03%).Conclusion This method is simple,reliable and Reproducible,can be used for quality control of the Gegen Qinlian pel ets.
作者
何欣欣
HE Xin-xin(Zhejiang Province Taizhou Luqiao Chinese Medicine Hospital,Taizhou,Zhejiang 318050,China)
