摘要
目的 检测CD177在炎症性肠病患者外周血和肠黏膜组织中的表达,并探究其对中性粒细胞迁移能力的影响.方法 收集18例活动期克罗恩病患者、21例溃疡性结肠炎患者以及14例健康对照者的外周血和肠黏膜组织标本,使用流式细胞术检测CD177分子表达.并采用免疫磁珠法分离上述患者和健康对照者外周血中CD177+和CD177-中性粒细胞,用Transwell检测两群细胞迁移能力.结果 CD177+中性粒细胞的比例在活动期克罗恩病和溃疡性结肠炎患者外周血和肠黏膜组织中均显著高于健康对照者(P<0.05).使用免疫磁珠的方法,分离得到外周血高纯度的CD177+和CD177-中性粒细胞,Transwell检测发现CD177+中性粒细胞的迁移能力明显高于CD177-中性粒细胞(P<0.05),且炎症性肠病患者CD177+中性粒细胞的迁移能力亦明显高于健康对照者的CD177+中性粒细胞(P<0.05).结论 CD177在炎症性肠病患者中外周血和肠黏膜组织中表达明显升高,且CD177+中性粒细胞的迁移能力增加,提示CD177+中性粒细胞可能在肠黏膜炎症发生过程中起重要的免疫调节作用.
Objective To examine CD 177 expression in peripheral Wood and inflamed mucosa from active patients with inflammatory bowel disease(IBD),and explore its potential role in regulating the migration of neulrophils.Methods Peripheral blood and inflamed intestinal mucosa were harvested from patients with active Crohr/s disease(n=18),active ulcerative colitis(n=21)and healthy controls(n=14).CD177 expression was determined by flow cytometry.CD177+and CD177-neutrophils were also isolated from peripheral blood of patients with active IBD by irnmunornagnetic beads,and the migration of these neutrophils was analyzed by Trails well.Results CD 177 expression was highly increased in peripheral blood neutrophils and inflamed mucosa from patients with active IBD compared with that in healthy controls(P<0.05).Purified CD177+and CD177'neutrophils were isolated from active IBD patients,and a Transwell analysis revealed that the migration capacity of CD177+neutrophils was markedly increased than that of CD177-neutrophils in all groups(P<0.05).Moreover,the migration capacity of CD177+neutrophils from IBD patients was also markedly increased compared with that from healthy controls(P<0.05).Conclusion Our data demonstrate tliat CD 177 expression is increased in patients with IBD,and that CD 177 promotes neutrophil migration and homing into intestinal mucosa.CD177+neutrophils may play an imporlanl regulatory role in modulating intestinal mucosal inflammation.
作者
周广玺
杨文静
余天名
刘占举
Zhou Guangxi;Yang Wenjing;Yu Tianming;Liu Zhanju(Department of Gastroenterology,The Shanghai Tenth People's Hospital of Tongji University,Shanghai 200072,China)
出处
《中华炎性肠病杂志（中英文）》
2017年第1期37-40,共4页
Chinese Journal of Inflammatory Bowel Diseases
基金
国家自然科学基金重点项目(816300017).
