摘要
Objective:To isolate,screen and analyze bacteria from different areas of Pakistan for the production of antimicrobial compounds,zinc solubilization and bioplastic production.Methods:Isolation and purification was proceeding with streak plate method.Antagonistic assay was completed with well diffusion and thin-layer chromatography.In vivo analysis of bioplastic was analyzed with Nile blue fluorescence under UV and Sudan staining.Results:A total of 18 bacterial strains purified from soil samples while 148 strains form stock cultures were used.Out of 166 only 94 showed antimicrobial activity against each of Grampositive and Gram-negative;cocci and rods.In case of heavy metal(ZnO and Zn_(3)(PO_(4))_(2)·4H_(2)O)solubilization,54 strains solubilized ZnO and 23 strains solubilized Zn_(3)(PO_(4))_(2)·4H_(2)O,while 127 strains grown on polyhydroxyalkanoate detection meedia supplemented with Nile blue medium showed bioplastic production by producing fluorescence under UV light.Four bacterial strains(coded as 100,101,104 and 111)were selected for further characterization.Induction time assay showed that strains 101,104,and 111 showed inhibitory activity after 4 h of incubation while strain 100 showed after 8 h.All four strains were tolerable to the maximum concentration of ZnO.Amplified products of both 16S rRNA and PhaC gene fragments of strain 111 were sequenced and submitted to GenBank as accession numbers EU781525 and EU781526.Conclusions:Bacterial strain Pseudomonas aeruginosa-111 has potential to utilize as biofertilize and bioplastic producer.
基金
Supported by International foundation for Science IFS Sweden(Grant No.F/4739-2F)and University of the Punjab,Lahore,Pakistan.
