摘要
Background:We sought to clarify the prevalence of occult hepatitis B virus(HBV)infection(OBI)and to determine whether OBI affects the surgical outcomes in curatively resected Japanese patients with hepatitis C virus(HCV)-related hepatocellular carcinoma(HCC).Methods:A total of 257 patients with HCV-related HCC who underwent curative surgical resection were enrolled.All enrolled patients were serologically negative for HBV surface antigen and positive for HCV antibody.DNA was extracted from formalin-fixed paraffin-embedded liver tissue.OBI was determined by the HBV-DNA amplification of at least two different sets of primers by TaqMan real-time polymerase chain reaction.Surgical outcomes were evaluated according to overall survival(OS),disease-specific survival(DSS),and disease-free survival(DFS).Results:OBI was identified in 15 of the 257(5.8%)cases.In the multivariate analyses,the factors significantly correlated with OS were BMI>25(P=0.0416),portal vein invasion(P=0.0065),and multiple tumors(P=0.0064).The only factor significantly correlated with DSS was T-stage(P=0.0275).The factors significantly correlated with DFS were liver fibrosis(P=0.0017)and T-stage(P=0.0001).The status of OBI did not show any significant correlation with OS,DSS or DFS,but a weak association with DSS(P=0.0603)was observed.Conclusions:The prevalence of OBI was 5.8%in 257 cases of HCV-related HCC.Although a weak association between DSS and OBI was observed,and statistical analyses were limited by small number of OBI cases,no significant correlation between OBI and surgical outcomes was detected.
基金
This study was supported in part by Japan Society for the Promotion of Science(JSPS)KAKENHI(Grants-in-Aid for Scientific Research C)Grant Number JP16K08650.
