摘要
目的探讨miRNA-210是否通过调控STAT3表达来影响肺癌细胞株A549增殖及凋亡。方法选用肺癌细胞株A549,通过干扰慢病毒敲低miRNA-210表达水平,RT-PCR检测miRNA-210、STAT3基因表达水平,Western blot检测STAT3蛋白水平,CCK-8检测A549细胞增殖水平。结果肺癌A549细胞中miRNA-210、STAT3表达上调(P<0.01);敲低miRNA-210后,STAT3表达水平明显降低(P<0.01),Bax与Bcl-2比值明显升高(P<0.01),而A549细胞增殖被显著抑制(P<0.01)。结论 miRNA-210可能通过调控STAT3表达从而影响肺癌细胞A549增殖和凋亡。
Objective To investigate whether mi RNA-210 affects proliferation and apoptosis of lung cancer cell line A549 by regulating STAT3 expression.Methods The lung cancer cell line A549 was used to knock down the expression level of miRNA-210 by lentivirus. The expression levels of miRNA-210 and STAT3 were detected by RT-PCR. The level of STAT3 protein was detected by Western blot. The proliferation of A549 cells was detected by CCK-8.Results The expression of mi RNA-210 and STAT3 was up-regulated in lung cancer A549 cells(P<0.01). After knocking down miRNA-210,the expression level of STAT3 was significantly decreased(P<0.01),the ratio of Bax to Bcl-2 was significantly increased(P<0.01),and the proliferation of A549 cells was significantly inhibited(P<0.05).Conclusion The miRNA-210 may affect the proliferation and apoptosis of lung cancer cell A549 by regulating STAT3 expression.
作者
黄鑫
王波
HUANG Xin;WANG Bo(Department of Respiratory and Critical Care Medicine,Chengdu Fifth People′s Hospital,Chengdu 611130,China)
出处
《解剖学研究》
CAS
2020年第3期228-232,共5页
Anatomy Research
