摘要
该研究从烟台某水产养殖公司循环海水养殖系统(MRAS)的曝气生物滤器中成功分离出一株高效好氧反硝化菌,结合形态学特征和16S rRNA基因鉴定确定为海杆菌属,命名为Marinobacter sp.strain B108。采用单因素实验确定了Marinobacter sp.strain B108好氧脱氮最适条件,并明确其在最适条件下的生长特性、好氧反硝化性能及氮平衡特征。Marinobacter sp.strain B108具有高效的好氧反硝化性能,无异养硝化能力。该菌好氧反硝化脱氮最适条件为:碳源为CH_(3)COONa、C/N为16、盐度为30‰。以NO_(3)^(-)-N为底物时,Marinobacter sp.strain B108在最适条件下24 h对102.86 mg/L NO_(3)^(-)-N去除率为100%,TN去除率为98.89%;以NH_(4)^(+)-N为底物时,该菌在最适条件下24 h对100.08 mg/L NH_(4)^(+)-N去除率为55.85%,被去除NH_(4)^(+)-N全部转化为胞内氮,降解过程中未检测到NO_(3)^(-)-N和NO_(2)^(-)-N。氮平衡分析表明Marinobacter sp.strain B108对NO_(3)^(-)-N、NO_(2)^(-)-N和NH_(4)^(+)-N的同化率分别为36.53%、38.80%和50.47%,以气体形式造成的氮损失率分别为62.48%、47.55%和1.12%。Marinobacter sp.strain B108的硝酸盐还原途径包括同化还原途径和好氧反硝化的异化还原途径,不具有异化还原成铵的代谢途径,氮的去除以好氧反硝化的异化还原为主。Marinobacter sp.strain B108在循环海水养殖废水硝酸盐处理方面具有良好的应用前景。
A high-efficient aerobic denitrifier was successfully isolated from the biological aerated filter of the marine recirculating aquaculture system(MRAS)belonging to an aquaculture company in Yantai.The isolated strain was identified as Marinobacter and termed Marinobacter sp.strain B108 based on its morphological characteristics and 16S rRNA analysis.The optimal factors affecting the nitrogen removal performances of Marinobacter sp.strain B108 were determined through single-factor experimental analysis.The growth characteristics,aerobic denitrification performance and nitrogen balance were also clarified under the optimal conditions.Marinobacter sp.strain B108 has high-efficient aerobic denitrification performance,and no heterotrophic nitrification capability.The optimal factors were CH_(3)COONa as carbon source,C/N ratio 16,salinity 30‰.The NO_(3)^(-)-N and TN removal efficiencies of Marinobacter sp.strain B108 under the optimum conditions in 24 hours were 100%and 98.89%with nitrate(102.86 mg/L)as the substrate,respectively.The NH_(4)^(+)-N removal efficiency under the optimum conditions in 24 hours was 55.85%.The NH_(4)^(+)-N removed was completely converted into intracellular nitrogen,and NO_(3)^(-)-N and NO_(2)^(-)-N were not detected during the degradation process.Nitrogen balance analysis showed that the assimilation rates of NO_(3)^(-)-N,NO_(2)^(-)-N and NH_(4)^(+)-N by Marinobacter sp.strain B108 were 36.53%,38.80%and 50.47%,respectively.At the same time,the rates of nitrogen loss in the form of gases were 62.48%,47.55%and 1.12%,respectively.The nitrate reduction metabolic pathway of Marinobacter sp.strain B108 comprise the assimilatory nitrate reduction to ammonium and dissimilatory nitrate reduction based on aerobic denitrification,not include the dissimilatory nitrate reduction to ammonium.The nitrogen removal is mainly performed through aerobic denitrification.Marinobacter sp.strain B108 shows a broad application prospect in nitrate treatment of marine recirculating aquaculture.
作者
王娇
林久淑
王文鑫
王福海
穆明浩
洪波
季军远
WANG Jiao;LIN Jiushu;WANG Wenxin;WANG Fuhai;MU Minghao;HONG Bo;JI Junyuan(Key Laboratory of Marine Environment and Ecology of Ministry of Education,Ocean University of China,Qingdao 266100,China;Shandong Key Laboratory of Marine Environmental Geology Engineering,Ocean University of China,Qingdao 266100,China;Innovation Research Institute,Shandong Hi-speed Group,Jinan 250001,China)
出处
《环境科学与技术》
CAS
CSCD
北大核心
2023年第8期134-143,共10页
Environmental Science & Technology
基金
国家自然科学基金项目(51978636)
关键词
好氧反硝化
循环养殖
脱氮性能
环境因子
氮平衡
aerobic denitrification
recirculating aquaculture
nitrogen removal performance
environmental factors
nitrogen balance analysis
