摘要
目的:探讨HSVGFP在宫颈癌循环肿瘤细胞(CTCs)中的诊断效果及临床治疗指导价值。方法:选择2018年4月-2019年10月宫颈癌患者82例作为对象,设为观察组;所有患者均经手术病理组织检查确诊,且患者确诊后给予手术治疗;选择同期健康体检者50例,设为对照组。所有患者入院后均采集外周静脉血4 mL,采用感染人的肿瘤细胞并能表达绿色荧光蛋白(GFP)的重组单纯疱疹病毒(HSVGFP)完成宫颈癌循环细胞检测,查阅观察组病历资料,包括:年龄、肿瘤直径、分化类型、淋巴结转移、肿瘤分期、病理类型,分析不同病理状态下CTCs阳性率,并完成多因素Logistic分析;绘制受试者工作特征(ROC)曲线,分析HSVGFP在宫颈癌CTCs中的诊断效能。结果:观察组宫颈癌患者82例CTCs检测阳性38例,阳性率为46.34%,高于对照组健康体检者(3例阳性,阳性率为6.00%)(P<0.05);观察组宫颈癌患者的CTCs阳性率在不同年龄、病理类型中的比较无统计学意义(P>0.05),在不同肿瘤直径、分化类型、肿瘤分期,及是否有淋巴结转移中的比较具有统计学意义(P<0.05);多因素Logistic分析结果表明:观察组宫颈癌患者中CTCs阳性率与肿瘤直径、分化类型、淋巴结转移、肿瘤分期具有统计学意义(P<0.05)。ROC曲线结果表明:HSVGFP在宫颈癌循环肿瘤细胞中ACU值为0.913,诊断敏感性为89.45%,诊断特异性为71.27%。结论:循环肿瘤细胞在宫颈癌患者中具有较高的表达,将HSVGFP用于宫颈癌循环肿瘤细胞测定中能获得较高的诊断效果,能指导临床治疗,值得推广应用。
Objective:To investigate the diagnostic efficacy and clinical value of HSVGFPin cervical cancer circulating tumor cells(CTCs).Methods:Eighty-two cervical cancer patients from April 2018 to Oc-tober 2019 were selected as the observation group.All patients were diagnosed by pathological exami-nation,and the patients were given surgical treatment after diagnosis.Fifty patients with healthy phys-ical examination during the same period were selected as the control group,and 4 mL of peripheral ve-nous blood were collected after admission.The circulating cell detection of cervical cancer was per-formed using recombinant herpes simplex virus(HSVGFP)that infects human tumor cells and expresses green fluorescent protein.The pathological data of the observation group,including age,tumor size,differentiation type,lymph node metastasis,tumor staging,and pathological type.The positive rates of peripheral CTCs under different pathological classifications were studied by complete multi-factor logistic analysis,and ROC curve was drew to analyze the diagnostic value of HSVGFPin cervical can-cer CTCs.Results:In the observation group,38 cases of cervical cancer were positive in CTCs,with a positive rate of 46.34%,which was higher than that of healthy controls(3 positive cases with a posi-tive rate of 6.00%)(P<0.05).In the observation group,the positive rate of CTCs did not change ac-cording to age and pathological type(P>0.05);but significant changes of CTCs positive rate were found between different subgroups of tumor diameter,differentiation type,lymph node metastasis,and tumor staging(P<0.05).Multivariate logistic analysis showed that tumor diameter,differentia-tion type,lymph node metastasis,and tumor staging were correlated to the positive rate of CTCs in cervical cancer patients of the observation group(P<0.05).The ROC curve results showed that HSVGFPin cervical cancer circulating tumor cells had an AUC value of 0.913,a diagnostic sensitivity of 89.45%,and a diagnostic specificity of 71.27%.Conclusion:Circulating tumor cells h
作者
阎蓓
邸石
周广
刘宾磊
YAN Bei;DI Shi;ZHOU Guang;LIU Binlei(Wuhan Third Hospital&Tongren Hospital of Wuhan University,Wuhan 430060,Hubei,China;Hubei University of Technology,Wuhan 430068,Hubei,China)
出处
《武汉大学学报（医学版）》
CAS
2020年第2期235-239,共5页
Medical Journal of Wuhan University
基金
湖北省卫生计生委科研基金资助项目(编号:WJ2017F020).
关键词
循环肿瘤细胞
宫颈癌
绿色荧光蛋白
重组单纯疱疹病毒
诊断效果
Circulating Tumor Cells
Cervical Cancer
Green Fluorescent Protein
Recombinant Herpes Simplex Virus
Diagnostic Value
