摘要
抗生素的滥用导致医院获得性感染问题日益突出,光动力抗菌化学疗法深负众望。采用高压静电纺丝法制得原位包埋竹红菌素的P(MMA-co-MAA)纳米纤维膜,后将亚甲基蓝吸附结合到P(MMA-co-MAA)纳米纤维膜表面得到双光敏剂负载的纳米纤维膜,利用扫描电子显微镜、傅里叶红外光谱、紫外-可见漫反射和热失重等手段表征其微观形貌、化学结构以及热学性质,借助碘化钾底物氧化实验、光动力抗菌测试评价了光敏剂负载后对纤维膜光动力行为的影响。结果表明:包埋负载竹红菌素以及表面吸附负载亚甲基蓝对P(MMA-co-MAA)的形貌及热学性质影响较小;表面负载亚甲基蓝的纳米纤维膜的光动力性能优于包埋负载竹红菌素的纳米纤维膜,且略优于共负载的纳米纤维膜。由此推断,基于单线态氧扩散距离的局限性,表面负载的固定方式优于包埋负载。
The abuse of antibiotics leads to the increasing problem of hospital acquired infection,and photodynamic antimicrobial chemotherapy is expected to solve the problem.P(MMA-co-MAA)composite nanofibrous membranes were prepared by high-voltage electrospinning with embedding in situ of hypocrellin,then the methylene blue was adsorbed to P(MMA-co-MAA)to obtain double photosensitizer-loaded nanofibrous membrane material.The surface morphology,chemical structure and thermal properties of P(MMA-co-MAA)loaded with photosensitizer were studied by scanning electron microscopy,fourier transform infrared spectroscopy,UV-Vis diffuse reflection and thermogravimetric analysis.The effect of photosensitizers loading on the photodynamic behavior of the membranes was evaluated by potassium iodide substrate oxidation test and photodynamic antimicrobial test.The results showed that the morphology and thermal properties of P(MMA-co-MAA)were less affected by the embedment of hypocrellin and the surface adsorption of methylene blue.And the photodynamic properties of the membranes which absorbed methylene blue were better than those embedded hypocrellin and slightly better than those co-coated the two.Presumably,the fixed surface load was superior to the embedded load based on the limitations of singlet oxygen diffusion distance.
作者
韩家宝
潘崎江
饶丽婷
王玉梅
王婷婷
王清清
Han Jiabao;Pan Qijiang;Rao Liting;Wang Yumei;Wang Tingting;Wang Qingqing(Key Laboratory of Eco-textile(Jiangnan University),Ministry of Education,Wuxi 214122)
出处
《化工新型材料》
CAS
CSCD
北大核心
2019年第S01期36-40,共5页
New Chemical Materials
基金
江南大学国家级大学生创新创业训练计划项目“创新训练项目”(201810295002)
国家自然科学基金青年基金(51603090)
中国博士后科学基金(2018M630516)
中央高校基本科研业务费专项资金(JUSRP51907A).
