摘要
为了克服单一材料性能上的局限性,以活性炭(AC)为原料,对AC进行氧化处理,使其表面获得含氧活性基团,应用原子转移自由基聚合(ATRP)法对AC进行表面改性得到接枝共聚物。通过与2-溴异丁酰溴(BiBB)反应获得ATRP引发剂,室温下以溴化亚铜(CuBr)为催化剂、2,2-联吡啶(bpy)为配体、甲醇/水为溶剂、对苯乙烯磺酸钠(SStNa)为单体,通过ATRP法得到活性炭接枝对苯乙烯磺酸钠(AC-g-PSStNa)复合材料。复合材料兼具无机粒子的化学稳定性以及接枝聚合物的多样性。通过傅里叶变换红外光谱仪、扫描电子显微镜、X射线光电子能谱仪、热重分析仪对制备的接枝材料进行表征。利用静电吸附法对辣根过氧化物酶进行固定,用单因素变量法考察固载酶和游离酶在不同温度、pH条件下的相对活性和苯酚降解率。结果表明:与游离酶相比,固载酶具有更好的热稳定性,其在60℃时对苯酚依然有65%的降解率,说明固载酶可以在较大的温度梯度内发挥催化作用。pH=8时,固载酶对苯酚的降解率最大,达到78%,在偏碱性条件下固载酶有较高的降解率。
In order to overcome the limitation of the performance of single material,activated carbon(AC)was used as raw material to oxidize AC to obtain oxygen-containing active groups on its surface.The surface of AC was modified by atom transfer radical polymerization(ATRP)method.A graft copolymer was obtained.The ATRP initiator was obtained by reacting withα-bromoisobutylacyl bromide(BiBB).At room temperature,cuprous bromide(CuBr)was used as the catalyst,2,2-bipyridine(bpy)as the ligand,and CHOH/HO as the sovent.The AC-g-PSStNa(activated carbon@sodium p-styrenesulfonate)composite was obtained by ATRP polymerization at room temperature using sodium p-styrene sulfonate(SStNa)as the solvent.The composite material combined the chemical stability of inorganic particles with the diversity of graft polymers.The prepared grafted materials were characterized by Fourier transform infrared spectroscopy,scanning electron microscopy,X-ray photoelectron spectroscopy,and thermogravimetric analyzer.The horseradish peroxidase(HRP)was immobilized by electrostatic adsorption,and the relative activities and phenol degradation rates of the immobilized enzyme and the free enzyme at different temperatures and pH were investigated by the single-factor variable method.The results showed that compared with the free enzyme,the immobilized enzyme had better thermal stability,and it still had a 65%degradation rate of phenol at 60℃,indicating that the immobilized enzyme could play catalytic role in large temperature gradient.When pH=8,the degradation rate of phenol by the immobilized enzyme was the largest,reaching 78%,and the immobilized enzyme had higher degradation rate under the alkaline condition.
作者
毕淑娴
郝红英
侯光晖
孙恬恬
詹海鹃
刘万毅
舒士倡
Bi Shuxian;Hao Hongying;Hou Guanghui;Sun Tiantian;Zhan Haijuan;Liu Wanyi;Shu Shichang(State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering,Ningxia University National Demonstration Center for Experimental Chemistry Education,College of Chemistry and Chemical Engineering,Ningxia University,Yinchuan 750021;Science and Chemical Engineering,Ningxia Institute of Technology,Shizuishan 753000)
出处
《化工新型材料》
CAS
CSCD
北大核心
2022年第S01期286-291,共6页
New Chemical Materials
基金
国家自然科学基金(21467022)
宁夏回族自治区重点研发计划沿黄试验区科技创新发展一般项目(2018BDE23008)
宁夏自然科学基金(022AAC 03046)
大学生创新创业项目(2021107490289
202210749382)
