摘要
为了探索牛肌肉高表达lnc23调控骨骼肌卫星细胞成肌分化的分子机制,试验在前期下调lnc23定量蛋白质组学的基础上,对差异表达蛋白进行KEGG富集分析,并筛选显著富集的与肌肉发育密切相关的信号通路,绘制lnc23调控成肌分化的潜在网络并采用实时荧光定量PCR和Western-blot技术进行验证。结果表明:lnc23下调后,有TNNC1、TNNC2、FN1、RPS6KA2、CAMK2D、THBS2、ARPC1A、CAMK1D、PFN2、ITGA6、PIK3CA、ITGB3、CAMK2G、PDGFRA、IGF2共15个显著下调的差异表达蛋白,富集于与肌肉发育密切相关的MAPK信号通路、PI3K-AKT信号通路、细胞骨架信号通路和Ca2+信号通路中。说明lnc23可能通过PDGFRA和IGF2介导的MAPK信号通路、整合素介导的PI3K-AKT信号通路、PFN-Arp2/3轴介导的细胞骨架信号通路以及TNNC与CAMK调控Ca2+转运等途径调控牛骨骼肌卫星细胞成肌分化。
To further explore the molecular mechanism of muscle high-expressed lnc23 in regulating the myogenic differentiation of bovine skeletal muscle satellite cells,KEGG enrichment analysis of differentially expressed proteins was performed,significantly enriched and closely related to muscle development signal pathways were screened,and the potential network of lnc23 regulating myogenic differentiation was drawn and verified by qRT-PCR and Western-blot on the basis of down-regulating the quantitative proteomics of lnc23 in prophase in this experiment.The results showed that a total of 15 differentially expressed proteins(TNNC1、TNNC2、FN1、RPS6 KA2、CAMK2 D、THBS2、ARPC1 A、CAMK1 D、PFN2、ITGA6、PIK3 CA、ITGB3、CAMK2 G、PDGFRA、IGF2)were enriched in MAPK signaling pathways,PI3 K-AKT signaling pathways,cytoskeleton signaling pathways,and Ca2+signaling pathways related to muscle development after knocking down lnc23.It is suggested that lnc23 might regulate myogenic differentiation of bovine skeletal muscle satellite cells through MAPK signal pathway mediated by PDGFRA and IGF2,PI3 K-AKT signal pathway mediated by integrin,cytoskeleton remodeling mediated by PFN-Arp2/3 axis,and Ca2+transport regulated by TNNC and CAMK.
作者
牛慧慧
陈明明
张林林
郭益文
李新
丁向彬
郭宏
NIU Huihui;CHEN Mingming;ZHANG Linlin;GUO Yiwen;LI Xin;DING Xiangbin;GUO Hong(Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300384,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2020年第21期7-14,共8页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金面上项目(31572380)
