摘要
目的:比较异丙酚、七氟醚对肝缺血再灌注大鼠肝肾功能、心肌功能及脑损伤的影响。方法:选取清洁级成年雄性SD大大鼠100只为实验材料,其中随机选取75只建立肝缺血再灌注模型,并根据处理方式均分为缺血再灌注组(B组)、异丙酚组(C组)、七氟醚组(D组),另25只为假手术组(A组)。于再灌注即刻采集血样,检测天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、肌酐(Cr)、尿素氮(BUN)水平,评价肝肾功能。于再灌注30min将大鼠处死,取心肌组织检测心肌细胞凋亡率与心肌磷酸化蛋白激酶B(p-Akt)、总蛋白激酶B(t-Akt)、Bcl-2表达量,用于评估心肌功能。并断头取脑组织,检测神经元特异性烯醇化酶(NSE)、S100B蛋白含量,用于评估脑损伤情况。结果:B、C、D组肝缺血再灌注后即刻采集血样检测血清AST、ALT、Cr、BUN水平均显著高于A组。B组心肌细胞凋亡率及p-Akt、t-Akt、Bcl-2含量高于A、C、D组,且C组心肌细胞凋亡率、p-Akt、Bcl-2含量显著低于D组。B组NSE、S100B蛋白水平高于A、C、D组,且C组NSE、S100B蛋白水平低于D组。结论:异丙酚与七氟醚均可减轻肝缺血再灌注大大鼠的心肌损伤与脑损伤程度,但异丙酚效果更显著,两药对肝肾功能影响基本相当。
Objective To compare the effect of propofol and sevoflurane on liver and kidney function,myocardial function and brain injury in mice with hepatic ischemia-reperfusion injury.Methods A total of 100 male mice were selected,and 75 of them were randomly selected to make the ischemia-reperfusion model,and were furtherly divided into three groups:the ischemia-reperfusion group(group B),propofol group(group C)and sevoflurane group(group D).The rest were assigned to the sham-operated group(group A).Blood samples were collected immediately after reperfusion to detect the levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT),creatinine(Cr),urea nitrogen(BUN)and evaluate the liver and kidney function.Mice were killed 30 minutes after reperfusion.Myocardial apoptosis rate and expression of phosphorylated protein kinase B(p-Akt),total protein kinase B(t-Akt)and Bcl-2 were measured in myocardial tissues to evaluate myocardial function.The brain tissues were cut off and the contents of neuron specific enolase(NSE)and S100B protein were detected to evaluate the brain injury.Results Serum AST,ALT,Cr and BUN levels were detected immediately after hepatic ischemia reperfusion in group B,C and D,which were significantly higher than those in group A.The apoptosis rate and the contents of p-akt,t-akt and bcl-2 in group B were higher than those in groups A,C and D,and the apoptosis rate,p-akt and bcl-2 in group C were significantly lower than those in group D.The levels of NSE and S100B protein in group B were higher than those in group A,C and D,and the levels of NSE and S100B protein in group C were lower than those in group D.Conclusion Both propofol and sevoflurane can alleviate myocardial and brain injury in mice with hepatic ischemia-reperfusion injury,but propofol has bettert effect.The effects of propofol and sevoflurane on liver and kidney function are basically the same.
作者
杨柳
汪晶晶
谭礼强
黄鹏翰
谢易租
王艳冰
Yang Liu;Wang Jing-jing;Tan Li-qiang;Huang Peng-han;Xie Yi-zhao;Wang Yan-bing(Anesthesia,Bishan Hospital,the First Affiliated hospital of Chongqing Medical University,Chongqing 402760,China)
出处
《湖南师范大学学报（医学版）》
2020年第4期77-80,共4页
Journal of Hunan Normal University(Medical Sciences)
关键词
肝缺血再灌注
异丙酚
七氟醚
心肌损伤
脑损伤
hepatic ischemia-reperfusion
propofol
sevoflurane
myocardial damage
brain damage
