摘要
We established a f uorescence“turn-on”sensing platform for glutathione(GSH)detection utilizing chitosan-based glutaraldehyde non-conjugated polymers(GCPF)as nanomaterials via f uorescence resonance energy transfer(FRET)principle.Owing to the overlapping property of absorption spectrum of MnO^(2)nanosheets with f uorescence spectrum of GCPF,f uorescent intensity of GCPF was quenched in the presence of MnO^(2)nanosheets because of FRET principle.When GSH was added,MnO^(2)nanosheets were reduced and decomposed into large amounts of Mn^(2+)ions owing to reducing property of GSH.Accordingly,the quenched f uorescent intensity was turned on again.Therefore,this platform based on MnO^(2)nanosheet has been f rst applied to f uorescence“turn-on”detection of GSH from 0.5 to 50μmol/L with sdetection limit of 84 nmol/L.It showed high selectivity in GSH detection towards other ions and biomolecules such as L-lysine,L-threonine,L-valine,L-glutamic acid and DL-aspartic acid.When it was utilized in detecting GSH in serum samples,satisfactory recoveries ranged from 101.5%to 103.2%,indicating the accuracy of this f uorescence“turn-on”platform in bioanalysis.
基金
financially supported by Chongqing Research Program(cstc2018jcyjAX0742)of Basic Research
Frontier Technology,Scientific and Technological Research Program(KJQN201900521)of Chongqing Education Committee
Scientific Research Fund(2019YJ0307)of Sichuan Provincial Science and Technology Department
Dazhou Municipal Science Project(18YYJC0002)of Technology Bureau Application Foundation
