摘要
一、序言Rous和Murphy等[1,2]曾将Rous肉瘤组织及上清液接种至鸡胚胚膜及胚内,引起肝,心,胃及胸腔壁长瘤及肝脏出血。以后Keogh[3]报导Rous肉瘤活细胞于接种至鸡胚尿囊绒毛膜(CAM)后引起肝及肺产生大小瘤块;Okar-Blom[4]及Karnofsky等[5]分别将病毒注入胚膜均能引起胚胎肝脏出血.
The pathological changes of the visceral organs i.e.the liver,spleen,lung,meso-nephros and gonad of the Rous virus infected chick embryo have been investigated.The organ tissues were removed at various intervals from 6 hours to 10 days after virus inoculation.They were fixed in Carnoy’s solution and the sections were stained with methyl green-pyronin.Some of them were stained with Alcian blue-hematoxylin for acid muco-polysaccharide and toluidine blue for metachromatic reaction.Sequential histologi-cal examination on the process of pathological changes and their histochemical reactions of the visceral organs revealed the following facts:Hemorrhagic lesions appeared in 60.3%of inoculated host livers from the 5-7 days onward.Distinctive focal necrobiotic change of the liver cells,destruction of the endo-thelial cells,distention,hyperaemia and extravasation of the blood vessels and sinusoids which characterized the hemorrhagic lesions had been observed.Other pathological changes such as the distortion of the hepatic cords,nuclear pyknosis and loss of staining reaction of the liver cells in the hemorrhagic lesions were also found.Hemorrhagic lesions also appeared in the mesonephros,lung,intestine and heart of some of the em-bryos having received virus inoculation.In some of the embryos,focal sarcoma-like proliferation or tumor-like nodules were found in the liver,lung and mesonephros after 7-9 days of virus inoculation.Alcian blue staining of the nodules or foci demonstrated the presence of acid muco-polysaccharide reaction in the ground substance,indicating an early phase of growth of Rous sarcoma in the infected mesodermal tissues in these organs.It was observed that a certain number of pyroninophilic round cells occurred in all of the visceral organs of the host.They formed aggregates around the blood vessels or scattered in the mesenchymal tissues.Sometimes these cellular aggregates were quite ex-tensive,forming large patches.The incidence of these cellular aggregates or patches in the liver,spleen,lung,meseone
作者
薛社普
梁德才
SHIEH SHER-PU;LIANG DEH-TAI(Department of Experimental Morphology,Institute of Experimental Medicine,Chinese Academy of Medical Sciences)
出处
《解剖学报》
CAS
1965年第3期314-323,共10页
Acta Anatomica Sinica
