摘要
关于黑质有纤维投射到纹状体的问题,很早以前就有人因损毁纹状体后在黑质观察到有退行性细胞溃变而提出过[1]。但破坏黑质后用Nauta银染法常不能在纹状体内追踪到终末前变性[2-4]。而用改良的Fink-Heimer法在大白鼠[5]及猫[6]的纹状体中则可见到大量的终末前变性。
0.02~0.3μl of 33%HRP(SigmaⅥ)solution in sterile water were injected with a stereotaxic instrument into various sites of the caudate-putamen in 40 rats.The animals were perfused transaortically with 3%paraformatdehyde and 1%glutaradehyde in 0.1 M phosphate buffer solution(pH7.4).Frozen sections were dried in a container with POfor 2~4 hours and examined for fluorescence in the nucleus nigra.After which,the sections were processed for HRP reaction according to Mesulam’s method.A number of cells of substantia nigra were found to show both fluorescence and HRP-positive product,thus confirming the dopaminergic nature of the nigroneostiatum projection.Furthermore,the nigroneostriatum projection was found to be topographically organized,the ventro-medial part of compact zone of the substantia nigra projecting to the dorso-medial caudate-putamen,the dorso-medial part of compact zone to the ventral caudate-putamen,the ventro-middle of compact zone to 1be dorso-lateral and central caudate-putamen,the caudo-lateral part to the central caudate-putamen,the middle part of reticular zone to the lateral and central caudate-putamen,the lateral zone of substantia nigra to the caudal caudate-putamen.Some overlap existed.Strionigral fibers were also topographically organized,which terminated predominantly about the cells of reticular zone.These may form a feedback loop concerning with the modulation of the activity of the dopaminergic neurons in the substantia nigra.
作者
包新民
邝国陶
Bao Xinmin;Kuang Guotao(The Fourth Military Medical College)
出处
《解剖学报》
CAS
1981年第3期253-259,340,共8页
Acta Anatomica Sinica
