摘要
用2~2.2公斤的白兔22只,在脊髓腰。段一侧注射一定量的HRP,存活12小时至7日,在脊髓脑干长距离顺、逆运送的基础上研究了顺、逆行运送的速度及HRP在标记部位存留的时间。脑干中主要观察的顺行标记部位为背侧副橄榄核,逆行标记部位为红核及苍白中缝核。各核中顺行或逆行标记的出现及其消长过程相同,存活18小时后首先出现,2日后达高峰,7日后消褪殆尽。本研究得到以下的结论:1.HRP被顺、逆行运送的速度相等,每日至少350毫米,属快速运送。2.标记的净积累时间约为1日,故最佳存活期大致相当按纤维长度计所需的运输时间加1日。3.HRP在顺、逆行标记部位最多可存留~6日。4.HRP在不同纤维系的运速及存留时间相同。
It has been found by Ju(’81)that HRP could be anterogradely transported forlong distances.Based on this fact the rate of anterograde transport was investigatedin the present study,and,at the same time,the rate of retrograde transport and thetime of disappearance of HRP from the labeled sites were observed.Twenty tworabbits,weighing 2~2.2 kg,were used.The HRP was injected into the lower lumbarspinal cord unilaterally.The animals were sacrificed after 12 hours to 7 days andwere processed with benzidine and o-dianisidine.The anterogade labeling in the dorsalaccessory olivary nucleus and the retrograde labeling in the red nucleus and thenucleus raphe pallidus were chosen for study.It was quite unexpected to find that the anterograde and retrograde labelingsparalled each other in time as well as in intensity.They appeared at the same timeand waxed and waned practically in full accord.Labeling of the brainstem firstappeared at 18 hour’s survival,reached its peak at the 2nd day,and then graduallyfaded away till at the 7th day when only traces of labeling remained.In the casewhich showed the earliest labeling the distance between the injection site and theobex,which was at about the same level as the most densely labeled parts of thedorsal accessory olivary nucleus and nucleus raphe pallidus,was measured to be 265mm and the rate of transport,the same for anterograde and retrograde transports,was calculated to be more than 350mm per day.Counting from the time of theearliest labeling,HRP was found to remain at the labeled site for as long as 5~6 days.The period of net accumulation of HRP at the labeled sites was about 1 day.Thus,the best survival period would be roughly the time required for HRP to travel thefiber tract plus 1 day.Neurons of the red nucleus and the nucleus raphe pallidus differ greatly intheir morphology,physiology as well as biochemistry.The results in them however,were similar.This,in connection with observations on other nuclei,both anterogr-adely and retrogradely labeled,suggests that the rate of t
作者
鞠躬
舒斯云
李文明
郭峰
杜永樑
Ju Gong;Shu Siyun;Li Wenming;Guo Feng;Du Yongliang(Department of Anatomy,The Fourth Military Medical College)
出处
《解剖学报》
CAS
1983年第1期37-46,116-117,共12页
Acta Anatomica Sinica
