摘要
选择6例健康人(其中3例吸烟、3例无吸烟嗜好)为对象,研究棉酚诱发淋巴细胞SCE频率的影响。实验分为加或不加S-9的0.5,1,3,5,7μg/ml 5个棉酚剂量组,比较吸烟者与不吸烟者的各棉酚剂量组,诱发体外人体外周血淋巴细胞SCE频率的差异。结果表明除例2的1μg/ml,例3的1,7μg/ml等个别棉酚组SCE频率与各自的对照组比较,显示有统计学意义的改变(P<0.05)外,3例大部棉酚组的SCE频率变化,均无统计学意义(P>0.05)。用相同方法,改用二甲基亚砜作溶剂,对上述3例重复实验的结果,亦无显著差异(P>0.05)。有吸烟嗜好的3例正常人中,加S-9的各棉酚组SCE频率,差异非常显著(P<0.001)。不加S-9的各棉酚组,除少数棉酚组SCE频率有明显升高(P<0.001)外,大多数棉酚组SCE频率虽有变化,但无统计学意义的改变(P>0.05)。分析上述结果,棉酚对正常人无诱变作用。吸烟者3例,其SCE频率升高可能与人吸入烟凝结物中的苯并芘等致癌物与S-9相互作用形成环氧化物有关。
For this study 6 normal adults were chosen,3 of them were non-smokers and had no habit of drinking alcohol,they were divided into 0.5,1,3,5,7μg/ml gossypol groups with or without adding S-9 for activation.It was found that in 1μg/ml group(case 2)and in 1μg/ml,7μg/ml group(case 3),SCE frequencies showed significant changes(P<0.05)as compared with control groups.While the changes in all other dosage groups of case 1,2,3 had no statistic significance(P>0.05).When DMSO was used as solvent and repeat the same experiments,the results didn’t show any significant change(P>0.05).SCE frequency showed dramatic changes(P<0.001)in three smokers when S-9 was added.Without S-9,most of gossypol groups showed little change(P>0.05),except a few cases with increased SCE frequency(P<0.001).It was concluded,therefore,that gossypol had no inducing effect on SCE frequency in human cells.The increased frequency of SCE in three smokers might be due to the high level of aryl hydrocarbon hydroxylase(AHH).
作者
蔡有余
刘裕
韩香玉
须昌隆
马林茂
罗会元
薛社普
Cai Youyu;Liu Yu;Han Xiangyu;Xu Changlong;Ma Linmao;Luo Huiyuan;Xue Shepu(Basic Medical Institute,Chinese Academy of Medical Sciences,Beijing)
出处
《解剖学报》
CAS
1985年第2期209-216,共8页
Acta Anatomica Sinica
关键词
棉酚
SCE频率
S-9组份
致突、致癌或致畸效应
Gossypol
Sister chromatid exchange frequency
S-9 fraction
Mutagenic
Carcinogenic or teratogenic effect
