摘要
本文用特异的抗鼠促黄体激素(抗LH)血清,以PAP免疫组织化学法显示促性腺激素细胞(LH细胞),并用形态计量学方法研究注射大剂量促黄体激素释放激素类似物(LHRH-A)后,受孕大鼠促性腺激素细胞的数量、体积和核质比参数的变化。察表明注射大剂量LHRH-A后24小时,促性腺激素细胞的数目减少,细胞变小,核质比增大。每mm^(3)垂体组织中的细胞数由(4.254±0.21)×10^(4)个减少到(2.49±0.14)×10^(4)个;细胞体积由1295±35μm^(3)减到895±31μm^(3);核质比由0.144±0.005加大到0.229±0.010。注射后48小时,这些变化已经开始恢复。注射后168小时各参数均恢复。我们认为,注射大剂量LHRH-A后,受孕大鼠促性腺激素细胞的变化是由于外源性大剂量LHRH-A刺激促性腺激素细胞,使其释放大量LH,大剂量LHRH-A对促性腺激素细胞的影响是可恢复的。
The specific anti-rat LH in the peroxidase anti-peroxidase(PAP)immunohistochemical method was used to demonstrate the pituitary gonadotropic cells in the pregnant rats,and then the morphometric analysis method was used to study the change of the number,size and nucleo-cytoplasmic ratio parameters of the gonadotropic cells in pregnant rats after being injected by a high dose of LHRH-A.It was observed that on 24h after injection the number of gonadotropic cells decreased,their size became smaller and their nucleo-cytoplasmic ratio larger:the cell number per mm^(3) pituitary tissue was from(4.25±0.21)×10^(4) to(2.49±0.14)×10^(4);the cell size from 1295±35μm^(3) to 895±31μm^(3);the nucleo-cytoplasmic ratio from 0.144±0.005 to 0.229±0.010.On 48h after injection these changes were in the process of recovery.On 168h after injection all parameters were recovered.Our observations suggest that after being injected by a high dose of LHRH-A the change of gonadotropic cells in pregnant rats results from the exogenous high dose of LHRH-A which has stimulated gonadotropic cells to release a great amount of LH.It is believed,however,that the changes of gonadotropic cells caused by a high dose of LHRH-A can still return to normal.
作者
孙敏之
郑富盛
李伟雄
傅爱华
Sun Minzhi;Zheng Fusheng;Li Weixiong;Fu Aihua(Department of Histology and Embryology,Beijing Medical University,Beijing;Analyzing and Computing Center,Beijing Medical University,Beijing;Department of Physiology,Beijing Medical University,Beijing)
出处
《解剖学报》
CAS
1988年第2期187-191,235,共6页
Acta Anatomica Sinica
