摘要
本文报道假孕大鼠子宫内膜,在大剂量促黄体生成素释放激素类似物(LH-RH-A)作用下的组织学变化。用29只Wistar雌性大鼠,人工方法产生假孕蜕膜反应。从假孕第5天,每日腹部皮下注射LH-RH-A 200μg(溶于0.2ml生理盐水),连续4天。对照组注射等量生理盐水。于假孕第9、11、13天取材,经不同固定液固定后,做HE及显示结缔组织纤维和子宫内膜颗粒细胞的染色。结果表明,大剂量LH-RH-A可使假孕大鼠子宫内膜蜕膜化提前终止,蜕膜退化,子宫内膜颗粒细胞明显减少。
The decidua of pseudopregnant rats and of those effected by high dosage of luteinizing hormone-releasing hormone analogue(LH-RH-A)were studied histologically.After inducement of pseudopregnancy,29 Wistar virgin rats were divided into experimental and control groups.The experimental group was injected with 200μg.of LH-RH-A subcutaneously daily for four days,while to the control group,physiological saline was injected instead.Both experimental and control animals were killed on the 9th,11th and 13th day of pseudopregnancy.The uteri were fixed with appropriate fixatives and paraffin sections were processed with HE,phloxine-tartrazine,Foots and aldehyde fuchsin staihing methods.On the 9th day decidua of the control rats are well developed with many endometrial granulated cells,while in the decidua of the experimental group,the decidual cells become pyknotic,and the granulated cells are barely seen.The results suggest that a high dosage of LH-RH-A advances the necrosis of decidua of the pseudopregnant rats.
作者
何晶
王懋蔚
He Jing;Wang Maowei(Department of Histology and Embryology,Beijing Medical University,Beijing)
出处
《解剖学报》
CAS
1988年第3期307-311,352,共6页
Acta Anatomica Sinica
关键词
促黄体生成素释放激素
假孕
蜕膜
基质细胞
子宫内膜颗粒细胞
大鼠
Luteinizing hormone releasing hormone analogue(LH-RH-A)
Pseudopregnancy
Decidua
Stromal cells
Endometrial granulated cells
Rats
