摘要
本文报道对64只豚鼠的血淋巴细胞,分别在电针、给予纳洛酮或外源性阿片样肽(OLP)等不同条件下,以PAP免疫组织化学法显示甲脑啡肽(MEK)、亮脑啡肽(LEK)及β-内啡肽(β-EP)的免疫反应(IR)。另对部分免疫组织化学标本,随后显示酸性非特异性酯酶(ANAE)。约96%的血淋巴细胞呈强弱不等的OLP阳性反应。甲脑啡肽强阳性反应淋巴细胞(MEK-IIRL)和β-EP强阳性反应淋巴细胞,与ANAE的点型淋巴细胞,均呈显著正相关;但LEK强阳性反应淋巴细胞与ANAE的点型淋巴细胞的相关不显著。电针豚鼠“足三里”穴后,MEK、LEK及β-EP的强阳性反应淋巴细胞计数均显著提高。将电针组、纳洛酮组和对照组的结果做比较,见3组间的OLP(包括脑啡肽和内啡肽)的强阳性反应淋巴细胞计数差别皆不显著。但当分别加10^(-7)mol/L MEK、LEK和β-EP体外孵育淋巴细胞后,纳洛酮对OLP强阳性反应淋巴细胞有抑制效应,纳洛酮组和电针组的OLP强阳性反应淋巴细胞计数均呈显著差异。在外加10^(-12)~10^(-3)mol/L MEK体外孵育淋巴细胞后,可见电针组比对照组在10^(-10)~10^(-3)mol/L浓度间MEK免疫反应呈现一个明显高峰,提示电针可能提高潜在未结合的MEK受体的活性。
The met-enkephalin(MEK),leu-enkephalin(LEK)andβ-endorphin(β-EP)immunoreactivities were detected in the circulating lymphocytes of 64 guinea pigs by PAP immunohistochemical method under the conditions of electroacupuncture,naloxone or exogenous opioid like peptides(OLP).Subsequently theα-naphthyl acetate esterase(ANAE)was demonstrated on partial immunostained lymphocyte samples.The results showed that the OLP immunoreactivity with different intensity was found in approximate 96%of the lymphocytes.There was a positive correlation between the ANAE-focal pattern lymphocytes and MEK-IIRL(intense immunoreactivity lymphocytes)orβ-EP-IIRL;whereas there was no significant correlation between the ANAE-focal pattern lymphocytes and LEK-IIRL.The MEK-IIRL,LEK-IIRL andβ-EP-IIRL were all increased markedly in the guinea pigs which underwent electroacupuncture at"Zusanli"points.There was no significant difference in MEK-IIRL,LEK-IIRL andβ-EP-IIRL,when the acupuncture group,naloxone group and control group were compared with each other.However,the LEK-IIRL,MEK-IIRL andβ-EP-IIRL could be depressed by naloxone,when the lymphocytes were preincubated with 10^(-7)mol/L of MEK,LEK orβ-EP in vitro.Hence,the naloxone group had a significant difference in MEK-IIRL,LEK-IIRL orβ-EP-IIRL when compared with the acupuncture group.A high peak of the lymphocyte MEK-IR among the range of 10^(-10)—10^(-8)mol/L of MEK preincubation in the acupuncture group when compared with the control group.It suggests that electroacupuncture may enhance activity of the unbound MEK receptor.
作者
吴景兰
王一菱
柴信美
王希涛
Wu Jinglan;Wang Yilin;Chai Xinmei;Wang Xitao(Department of Histology and Embryology,Henan Medical University,Zhengzhon)
出处
《解剖学报》
CAS
1988年第3期312-318,344,共8页
Acta Anatomica Sinica
关键词
针刺
淋巴细胞免疫
阿片样肽
非特异性酯酶
免疫组织化学
豚鼠
Acupuncture
Lymphocyte cell-mediated immunity
Opioid like peptide
Nonspecific esterase
Immunohistochemistry
Guinea pig
