摘要
目的:探讨LncRNA-00311与HOXC10信号通路的关系及其对口腔鳞癌侵袭转移的影响。方法:以HaCaT细胞和4种人口腔鳞癌细胞株(HN4、Cal27、HN30、HN6)为细胞模型,LncRNA-00311过表达病毒感染肿瘤细胞后,用RT-PCR、蛋白质印迹法等检查相关基因及蛋白表达情况,同时采用划痕实验检测肿瘤细胞侵袭迁移能力的变化。结果:LncRNA-00311过表达的HN6细胞的迁移能力显著降低,同时HOXC10的基因及蛋白表达显著下降,E-cadherin蛋白表达显著上调,N-cadherin和Vinmentin显著下调。结论:LncRNA-00311可能通过降低HOXC10表达,抑制HOXC10信号通路,进而抑制EMT,从而降低口腔鳞癌侵袭和转移。
Objective:To investigate the relationship between LncRNA-00311 and HOXC10 signaling pathway and its effect on invasion and metastasis of oral squamous cell carcinoma(OSCC).Methods:HaCaT cells and four human oral squamous cell lines(HN4,Cal27,HN30,and HN6)were used as cell models.After LncRNA-00311 overexpressed virus infected tumor cells,the expression of related genes and proteins were examined by RT-PCR and Western Blot,and the invasion and migration ability of tumor cells were detected by Wound Healing.Results:LncRNA-00311 over-expressed HN6 cells showed significantly decreased migration ability,while HOXC10 gene and protein expression were significantly decreased,E-cadherin protein expression was significantly up-regulated,and N-cadherin and Vinmentin were significantly down-regulated.Conclusion:LncRNA-00311 may reduce the expression of HOXC10 and inhibit the HOXC10 signaling pathway,thereby inhibiting EMT,invasion and metastasis of OSCC.
作者
任振虎
杨溪
张新宇
苏瑜
陈文鑫
王学谦
曹巍
胡传宇
REN Zhen-hu;YANG Xi;ZHANG Xin-yu;SU Yu;CHEN Wen-xin;WANG Xue-qian;CAO Wei;HU Chuan-yu(Department of Oral and Maxillofacial Surgery,Hainan West Central Hospital Hospital,Hainan Danzhou 571700,China;Department of Oral and Maxillofacial&Head and Neck Oncology,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China;Stomatology Center,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei Wuhan 430030,China.)
出处
《临床口腔医学杂志》
2020年第1期3-6,共4页
Journal of Clinical Stomatology
基金
(张志愿)院士工作站科研基金(2019)
上海交通大学医学院附属第九人民医院“种子基金”(JYZZ016)
上海市抗癌协会“雏鹰计划”(SACA-CY1B06)
美捷登青年科学家研究基金(MJR20180001).
关键词
口腔鳞癌
长链非编码RNA
HOXC10
上皮间质转化
Oral squamous cell carcinoma
Long non-coding RNA
HOXC10
Epithelial-mesenchymal transition(EMT)
