摘要
目的通过观察参附注射液对大鼠心脏骤停/心肺复苏(CA/CPR)模型的脑组织Nogo1-NgR通路的影响及大鼠脑神经功能的变化情况,研究参附注射液阻断Nogo1-NgR通路促进心肺复苏后大鼠神经功能恢复的机制。方式将制备成功CA/CPR模型大鼠随机分为4组:常规西医组、假手术组、拮抗剂组、参附组,每组30只,另选30只健康大鼠为空白对照组。空白对照组不实行CA/CPR,每日上下午各给予生理盐水(2.1 mL/kg)颈外静脉注射1次;常规西医组在CA/CPR时应用西药肾上腺素、多巴胺等及亚低温处理;其他各组在常规西医组处理的基础上于CPR后1 h开始给予颈外静脉注相应剂量(2.1 mL/kg)药物干预:假手术组予注射生理盐水,拮抗剂组使用Nogo拮抗剂,参附组使用参附注射液,每日2次,连续1周。常规西医组、拮抗剂组、参附组统称为用药组。各组分别于CPR后24 h、3 d、7 d随机选择10只大鼠进行神经功能评分,并采用Western Blot法观察NgR在各组大鼠中海马以及皮层的表达情况。结论Western Blot显示,在空白对照组的海马及皮层组织中均有少量NgR表达。在海马组织中,经过CA/CPR的4组大鼠NgR蛋白的表达在第3天时达到最高,第7天时已较前下降,其中参附组最低,与拮抗剂组同时间点相比具有统计学意义(P<0.05)。在皮层组织中,经过CA/CPR的4组大鼠NgR蛋白的表达在前3天呈现升高的趋势,假手术组在第7天继续升高,3个用药组在第7天时NgR的表达则较第3天有所下降,用药组中拮抗剂组在第7天时最低,但第7天时拮抗剂组、参附组与常规西医组比较均没有统计学意义(P>0.05)。神经功能评分中,空白对照组小鼠各时间点评分均为0,假手术组在各组评分中均最高;在用药组中,大鼠神经功能的变化随着用药时间的延长而改善,其中拮抗剂组在第3天时在3个用药组中改善最明显(P<0.05),但在第7天时参附组、拮抗剂组神经功能评分差别�
Objective To observe the effect of Shenfu Injection on Nogo1-NgR pathway in brain tissue of rats with cardiac arrest/cardiopulmonary resuscitation(CA/CPR)model and the changes of brain nerve function in rats and study the mechanism of Shenfu Injection blocking Nogo1-NgR pathway to promote the recovery of nerve function in rats after cardiopulmonary resuscitation.Methods CA/CPR model rats were randomly divided into four groups:conventional western medicine group,sham operation group,antagonist group and Shenfu Injection group.In each group,there were 30 rats.In addition,30 healthy rats were put into the blank control group.The blank control group was not given CA/CPR,and normal saline(2.1 mL/kg)was injected into external jugular vein once a day in the morning and afternoon.The routine western medicine group was treated with Western medicine such as adrenaline and dopamineand treated with mild hypothermia during CA/CPR.On the basis of routine western medicine treatment,the other groups were given corresponding dosage(2.1 mL/kg)of drug intervention by external jugular vein injection 1 hour after CPR.The sham operation group was injected with normal saline,the antagonist group was injected with Nogo antagonist,and Shenfu Injection was used in Shenfu group.Each group was injected twice a day for 1 week.Routine western medicine group,antagonist group and Shenfu Injection group were collectively called medication group.Ten rats in each group were randomly selected on 24 hours,3 and 7 days after CPR for neurological score,and Western blot method was used to observe the expression of NgR in hippocampus and cortex of rats in each group.Results Western blot showed a small amount of NgR expression in the hippocampus and cortex of the blank control group.In hippocampal tissue,the expression of NgR protein reached the highest level in CA/CPR group at 3 days,and decreased at 7 days.The lowest level was in Shenfu Injection group,which had statistical significance compared with that of the antagonist group at the same time point(
作者
邓海霞
陀鹏
吴瑞华
贾思
赵飞兰
黄德庆
梁道业
张荣臻
高玉广
钟云青
温志浩
DENG Haixia;TUO Peng;WU Ruihua;JIA Si;ZHAO Feilan;HUANG Deqing;LIANG Daoye;ZHANG Rongzhen;GAO Yuguang;ZHONG Yunqing;WEN Zhihao(The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning 530000,Guangxi,China;Guangxi University of Traditional Chinese Medicine,Nanning 530000,Guangxi,China)
出处
《辽宁中医杂志》
CAS
2020年第10期178-181,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金青年基金项目(81503410)
关键词
参附注射液
神经功能
NgR蛋白
Shenfu Injection
neurological function
NgRprotein
