摘要
目的观察补肾活血方对于绝经后体内的成骨细胞在增殖、分化以及矿化能力等方面的影响作用。方法试验用大鼠40只,随机分成对照组,补肾活血方低、中、高剂量组,制备含药血清。通过各组血清对成骨细胞进行干预;用流式PI单染法检测成骨细胞周期,微量酶标法检测ALP活性,茜素红钙染色钙化结节并计数。结果干预后,各剂量组与对照组比较,各剂量组G0/G1期、G2/M期显著下降,S期和G2/M+S期显著升高;碱性磷酸酶活性、钙化结节数各剂量组与对照组比较均有所升高,中、高剂量组高于对照组。结论补肾活血方可以通过延长原代成骨细胞的S期进而促进其增殖、分化和矿化功能。
Objective:To observe the effects of Bushen Huoxue decoction on proliferation,differentiation and mineralization of post-menopausal osteoblasts.Methods:Forty rats in the experiment were randomly divided into the control group and the low,medium and high dose groups of Bushen Huoxue decoction to prepare drug-containing serum.Osteoblasts were intervened by serum from each group;osteoblast cycle was detected by flow cytometry,ALP activity was detected by microenzyme method,calcified nodules were stained with alizarin red calcium and counted.Results:After the intervention,the G0/G1 phase and G2/M phase of each dose group decreased significantly,and the S phase and G2/M+S phase increased significantly in each dose group.Alkaline phosphatase activity and calcified nodules The number of each dose group was higher than that of the control group,and the middle and high dose groups were higher than the control group.Conclusion:Bushen Huoxue decoction can promote its proliferation,differentiation and mineralization by prolonging the S phase of primary osteoblasts.
作者
庞江娜
武永富
梁芳
王江泳
赵旭兰
范亚坤
周艳艳
张灿
PANG Jiangna;WU Yongfu;LIANG Fang;WANG Jiangyong;ZHAO Xulan;FAN Yakun;ZHOU Yanyan;ZHANG Can(Shijiazhuang First Hospital,Shijiazhuang 050000,Hebei,China;Shijiazhuang Third Hospital,Shijiazhuang 050000,Hebei,China)
出处
《辽宁中医药大学学报》
CAS
2020年第1期43-46,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
河北省医学科学研究重点课题(20150913).
关键词
补肾活血方
成骨细胞
绝经骨质疏松
细胞周期
Bushen Huoxue decoction
osteoblasts
menopausal osteoporosis
cell cycle
