摘要
拟利用纳米乳包载紫檀芪,以提升其稳定性及生物可给性。以乳清分离蛋白(Whey protein isolate,WPI)为乳化剂,利用高压微射流制备纳米乳。结果表明,当WPI质量浓度为2%,油相质量含量为5%时,粒径在180nm左右。紫檀芪的加入在一定浓度范围内不影响乳液颗粒粒径。所得乳液对环境条件具有一定相应项,70℃加热处理后乳液粒径最大;当pH值在WPI等电点(约5)附近时,乳液稳定性最差;盐离子浓度升高会导致平均粒径增大,但一定的加热处理(50℃)可提升乳液的盐稳定性。空白紫檀芪溶液的保留率随光照时间线性下降,第10d约为70%。而纳米乳中紫檀芪保留率则维持在89%~93%。体外消化实验显示,纳米乳中紫檀芪的生物可给性大于60%,比空白紫檀芪油溶液提升3倍。因此,乳清蛋白乳液可用于功能食品及饮料中以提高脂溶性功能组分紫檀芪的生物可利用性。
The nanoemulsion was used to encapsulate pterostilbene,in order to improve its stability and bioaccessibility.Nanoemulsions were prepared under high-pressure microfluidizer by using whey protein isolate(WPI)as the emulsifier.The results indicated that when WPI concentration and MCT content were 2%and 5%,respectively,particle size was around 180 nm.At a certain concentration range,incorporation of pterostilbene would not affect the particle size of emulsions.The obtained nanoemulsion was sensitive to environmental conditions.Heating treatment under 70℃caused the highest particle size.Emulsion had the worst stability when pH was close to the isoelectric point of protein.Particle size of emulsion droplets increased with increasing of NaCl concentration.Whereas,the emulsion stability against NaCl was improved by heating treatment(50℃).Retention rate of pterostilbene before encapsulation linearly decreased with longer lighting time,which was about 70%after 10 days.While it could be maintained at 89%~93%after encapsulation.Results from in vitro digestion demonstrated that bioaccessibility of pterostilbene was higher than 60%,three-times increasing compared to pterostilbene-oil solution.Whey protein isolate-based nanoemulsion can be utilized in functional foods and beverages to enhance the bioavailability of lipophilic pterostilbene.
作者
舒桥
冯皓
SHU Qiao;FENG Hao(School of Chemistry,Chemical Engineering and Life Sciences,Wuhan University of Technology,Wuhan 430070,China)
出处
《武汉理工大学学报》
CAS
北大核心
2019年第1期13-20,共8页
Journal of Wuhan University of Technology
基金
HA/RGD双受体介导的多靶点智能给药系统的构建及评价(51473130).
关键词
紫檀芪
乳清分离蛋白
纳米乳液
光稳定性
生物可给性
pterostilbene
whey protein isolate
nanoemulsion
light stability
bioaccessibility
