摘要
皮肤是人体最大的器官,其受到烧伤及深度伤害难以自愈,严重影响周围神经、血管等组织的功能。该文以聚乙烯醇(PVA)为基体材料,通过原位合成法引入掺硅羟基磷灰石(HA-Si),经过反复冻融制得PVA/HA-Si水凝胶。通过XRD,SEM,FTIR,CCK-8,免疫荧光以及荧光定量PCR等技术对材料进行理化表征以及生物相容性评价,探索材料浸提液对hUVECs细胞活性的影响,观测材料对hUVECs细胞形态以及血管化相关基因表达的影响。研究结果表明无机-有机复合技术制备的PVA/HA-Si材料,与纯PVA相比,细胞黏附能力显著提高,HA-Si的加入上调血管化相关因子VEGF、b-FGF和TGF-α的表达,对血管再生起到一定的促进作用。PVA/HA-Si水凝胶具有成为一种促血管化皮肤修复材料的潜能。
Skin is the largest organ of human body,which is difficult to heal itself after severe injuries and burns.A polyvinyl alcohol(PVA)/Si-doped hydroxyapatite(HA-Si)Hydrogel was prepared by in situ synthesis,gradual freezing-thawing and freeze-drying method.XRD,SEM,FTIR,CCK-8,immunofluorescence and fluorescence quantitative PCR technology were utilized to analysis the chemical behavior and cell compatibility of the hydrogel.Compared to PVA hydrogel,HASi modification promoted cell adhesion on its surface.The addition of HA-Si up-regulates the expression of vascularizationrelated factors,such as VEGF,b-FGF and TGF-α,which is beneficial to promote vascular regeneration.PVA/HA-Si hydrogel promotes further research to become a skin repair material that promotes vascularization.
作者
李宾斌
练辰希
王欣宇
刘家玮
邵锜
LI Bin-bin;LIAN Chen-xi;WANG Xin-yu;LIU Jia-wei;SHAO Qi(State Key Laboratory of AdvanceTechnology for Materials Synthesis and Processing,Wuhan University of Technology,Wuhan 430070,China;Biomedical Materials and Engineering Research Center of Hubei Province,Wuhan University of Technology,Wuhan 430070,China)
出处
《武汉理工大学学报》
CAS
北大核心
2020年第8期37-45,共9页
Journal of Wuhan University of Technology
基金
湖北省技术创新专项重大项目(2017ACA168)
武汉理工大学自主创新研究基金项目(2019-CL-B1-22)
关键词
掺硅羟基磷灰石
原位合成
水凝胶
血管化
Si-doped hydroxyapatite
in situ synthesis
hydrogels
angiogenesis
