摘要
目的探讨大麻二酚(cannabidiol,CBD)对高糖环境中小鼠足细胞系MPC5细胞凋亡的影响。方法将MPC5细胞分为对照组(Vehicle)、高糖组(high glucose,HG)和CBD组。CCK-8试剂盒检测MPC5细胞的增殖活性,流式细胞术检测MPC5细胞的凋亡率,Western blot检测裂孔膜蛋白Nephrin与凋亡相关蛋白Bax及Bcl-2的表达情况。结果与Vehicle组相比,HG组明显抑制MPC5增殖并促进其凋亡[(25.27±2.15)%vs.(8.43±0.70)%];而CBD干预显著促进高糖环境中MPC5增殖且抑制凋亡[(12.83±1.05)%vs.(25.27±2.15)%;P<0.05]。同时,CBD干预显著升高高糖环境中MPC5细胞Nephrin蛋白的表达[(0.77±0.06)vs.(0.32±0.10)],降低促凋亡蛋白Bax与抗凋亡蛋白Bcl-2的蛋白表达比[(1.02±0.09)vs.(12.30±0.09)]。结论CBD能缓解高糖诱导的MPC5细胞凋亡。
Objective To investigate the effect of cannabidiol(CBD)on podocytes MPC5 apoptosis induced by high glucose.Methods MPC5 cells were randomly divided into vehicle group,high glucose(HG)group and CBD group.CCK-8 method was used to access cell proliferation,flow cytometry was used to evaluate apoptotic rate,and western blot was used to investigate protein level of Nephrin and apoptosis-related proteins Bax and Bcl-2.Results Compared with the Vehicle group,HG significantly inhibited MPC5 proliferation and promoted MPC5 apoptosis[(25.27±2.15)%vs.(8.43±0.70)%],while CBD obviously promoted MPC5 proliferation and inhibited MPC5 apoptosis[(12.83±1.05)%vs.(25.27±2.15)%;P<0.05]in the high glucose environment.Meanwhile,CBD administration increased Nephrin expression[(0.77±0.06)vs.(0.32±0.10)],and reduced Bax/Bcl-2[(1.02±0.09)vs.(12.30±0.09);P<0.05].Conclusions CBD attenuates high glucose-induced MPC5 apoptosis.
作者
苏静华
焦婷
韩宁
苏静克
党瑞杰
李琳
张海松
SU Jinghua;JIAO Ting;HAN Ning;SU Jingke;DANG Ruijie;LI Lin;ZHANG Haisong(Health Medical Center,the Third Medical Center of Chinese PLA General Hospital,Beijing 100039,China;Oncology Division,Affiliated Hospital of Hebei University,Baoding 071000,China;Department of Nephrology,the Third Medical Center of Chinese PLA General Hospital,Beijing 100039,China;Medical School of Chinese PLA,Beijing 100853,China)
出处
《武警医学》
CAS
2022年第4期303-306,共4页
Medical Journal of the Chinese People's Armed Police Force
基金
中国博士后科学基金资助项目(2019T120977)
关键词
大麻二酚
高糖
足细胞
细胞凋亡
cannabidiol
high glucose
podocyte
cell apoptosis
