摘要
为筛选具有高固碳及高经济价值的小球藻(Chotrella sp.),本研究首先使用柱状反应器在BG11培养基中对13株小球藻进行培养,通过通入30%CO2测量13株藻的生物量、比生长速率和固碳速率,通过比较获得了可相对耐受高浓度CO2的藻株LAMB031、LAMB033和LAMB034。然后在培养基中分别通入空气、5%、10%、15%、20%的CO2培养9 d,分析了3株初选藻株的生物量、固碳速率、总脂、多糖和蛋白等。研究表明,通入CO2显著促进了初选3株小球藻的生长,提高了固碳速率,影响了小球藻主要生化成分的积累,但是3株小球藻在不同CO2浓度下生长及生化成分的积累是不同的。综合比较显示,藻株LAMB031、LAMB033和LAMB034通入CO2浓度分别为20%、5%、15%时生长及生化组成较理想,生物量分别为2.185、1.804和2.175 g/L,固碳速率分别是0.448、0.423和0.420 g·L-1·d-1,总脂含量分别是28.7%、29.0%和28.4%,蛋白含量分别是48.5%、53.4%和40.1%,多糖含量分别是8.94%、2.78%和7.42%。本研究结果为利用小球藻进行CO2固定提供了技术依据。
In order to screen Chlorella sp.strains with high carbon fixation rate and high economic value,13 Chlorella sp.strains were first cultured in BG11 medium using a column reactor in this study with their biomass yield and specific growth and carbon fixation rates measured by passing 30%CO2.The algal strains LAMB031,LAMB033 and LAMB034 which were relatively resistant to high concentrations of CO2 were obtained by comparison.The air containing 5%,10%,15%and 20%of CO2,respectively,was introduced into the culture medium and the three strains were cultured for 9 days with their biomass yield,carbon sequestration rate and total lipid,carbohydrate and protein contents analyzed.The results showed that the introduction of CO2 significantly promoted the growth of three strains,increased their carbon sequestration rate and affected their accumulation of major biochemical components.However,the growth and the accumulation of biochemical components of three strains of Chlorella sp.were different under different CO2.When the CO2 concentration was 20%,5%and 15%,respectively,the growth and biochemical components of the algal strains LAMB031,LAMB033 and LAMB034 were similar each other.However,their biomass yield was 2.185,1.804 and 2.175 g/L,respectively;their carbon fixation rate was 0.448,0.423 and 0.420 g/L/d,respectively;their total lipid content was 28.7%,29.0%and 28.4%,respectively;their protein content was 48.5%,53.4%and 40.1%,respectively;their carbohydrate content was 8.94%,2.78%and 7.42%,respectively.The results of this study provided a technical basis for the use of three strains of Chlorella sp.for CO2 fixation.
作者
马雪彬
李赟
朱葆华
沈含
耿升英
杨官品
潘克厚
MA Xue-Bin;LI Yun;ZHU Bao-Hua;SHEN Han;GENG Sheng-Ying;YANG Guan-Pin;PAN Ke-Hou(The Key Laboratory of Mariculture(Ocean University of China),Ministry of Education,Qingdao 266003,China;College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Fisheries and Aquaculture,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China)
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2019年第S02期29-36,共8页
Periodical of Ocean University of China
基金
国家重点研究发展计划项目(2016YFB0601001)资助.
关键词
小球藻
生物量
固碳
CO2浓度
总脂
多糖
蛋白
Chlorella sp.
biomass
carbon fixation
CO2 concentration
total lipid
carbohydrate
protein
