摘要
为实现活体小动物体内不同部位荧光药物代谢过程的同步实时监测,同时为实时分析小动物体内不同荧光药物的转运特性提供实验平台,本文设计了一套基于荧光的四通道小动物在体监测及药代分析系统。系统包括激光光源模块、光纤荧光探测模块和软件及控制系统模块3个组成部分。采用波长为488、655和785nm的多波长激光器作为光源、Y型光纤探头组作为探头,采用光谱仪作为检测工具。利用FITC、阿霉素、ICG等荧光药物标记小动物不同器官,产生的荧光通过光开关的切换分时复用光谱仪,四个通道分别采集不同部位的荧光信号。基于Lab Windows平台开发系统软件,并通过USB串口通信,实现上位机与仪器设备之间的通信。本系统可实现三种激光、多种荧光药物、四个通道的小动物在体监测,可以稳定地检测到荧光素钠溶液的荧光波峰,灵敏度达到了要求,基本满足活体小动物不同组织不同荧光药物代谢过程的实时监测需求。本系统为进一步研究纳米药物在活体小动物体内的转运特性提供了分析平台。
In order to achieve simultaneous real-time monitoring of fluorescent drug metabolism processes in different parts of in vivo small animals,and to provide an experimental platform for real-time analysis of the transport properties of different fluorescent drugs in small animals,a four-channel fluorescence-based in vivo monitoring and pharmacokinetic analysis system for small animals is designed in this paper.The system consists of three components:laser light source module,fiber optic fluorescence detection module and software and control system module.A multi-wavelength laser with wavelengths of 488,655and 785 nm was used as the light source,a Y-type fiber optic probe set as the probe,and a spectrometer as the detection tool.Fluorescent drugs such as FITC,adriamycin and ICG were used to label different organs of small animals,and the generated fluorescence was time-multiplexed through the switching of the optical switch to the spectrometer,and the fluorescence signals of different parts were collected in four channels.The system software is developed based on LabWindows platform,and the communication between the host computer and the instrumentation is realized through USB serial communication.The system can realize in vivo monitoring of small animals with three lasers,multiple fluorescent drugs and four channels,and can stably detect the fluorescence wave peak of sodium fluorescein solution with the required sensitivity,which basically meets the demand for real-time monitoring of different fluorescent drug metabolic processes in different tissues of living small animals.This system provides an analytical platform to further study the transport properties of nanomedicines in living small animals.
作者
陈超凡
周慧晶
王飞龙
李怡燃
钱志余
陶玲
CHEN Chaofan;ZHOU Huijin;WANG Feilong;LI Yiran;QIAN Zhiyu;TAO Ling(College of Automation engineering,Nanjing University of Aeronautics and Astronautics,Nanjing,Jiangsu,211106,China)
出处
《生命科学仪器》
2022年第4期39-45,共7页
Life Science Instruments
基金
江苏省重点研发计划(社会发展)项目(BE2020705)
江苏省自然科学基金(BK20190387)
关键词
纳米药物
荧光染料
多波长激光器
Y型光纤探头
同步监测
nanodrug
fluorescent dye
multi-wavelength laser
Y-type fiber probe
Simultaneous monitoring
