摘要
人T淋巴细胞与绵羊红细胞(SRBC)在不同条件下可形成各种类型的E花环。一般E花环的形成有着明显的温度依赖性[1]。其最适温度为4~24℃,在37℃30分钟快速解离。E花环的结合十分松脆,稍加振摇后即分散。
The aim of the present paper was to investigate conditions influencing the formation of E_(s),the possible mechanism of formation and its clinical significance.The method we employed was similar to the E-rosette test,except that at the end of incubation at 4℃for 2 hours,the tubes were thoroughly shaken and left at 37℃for 30 minutes before reading.Using this technique,we studied the conditions affecting the En formation and the value of E_(s)of PBL from 31 normal persons and from 159 patients with different diseases.The experimental results obtained were as follows:(1)the mean value of E_(s)of normal controls was 3.3±1.3%(31 cases),of thymocytes 73.3%(6 cases);(2)incubation of PEL with mitogens or specific antigens in vitro for 72 hours markedly increased the percentage of E_(s)-RFC,the figures being 51%for PHA(4 cases),26.6%for Con A(6 cases),12.3%for SK-SD(6 cases)and 12%for BCG(6 cases).The shape of the curves for PHA and for Con A were parallel with those of lymphooytio transformation test induced by these mitogens but were somewhat lower,and(3)finally,the results of E_(s)test on PBL from patients suffering from 8 differentMean Value of E,RFC of 159 Cases Suffering from S Types of Diseases types of diseases presumably associated with cellular immunity was tabulated below.Prom the above data,the appearance of E_(s)-RFC seemed to be the direct activation of T cells.If so,the test may be of value to evaluate the competence of cellular immunity of the patients suffering from those infections associated with mediation of cellular immunity,such as chronic active hepatitis,typhoid fever,leprosy,etc.,and possibly guidance on therapeutic measures.
出处
《上海医科大学学报》
1980年第1期1-10,共10页
Journal of Fudan University(Medical Science)
