摘要
研究建立了同时测定混合型饲料添加剂中肉桂醛、丁香酚、香芹酚和百里香酚含量的液相色谱方法。混合型饲料添加剂样品用甲醇提取,以C18柱(150 mm×4.6 mm×5μm)为分析柱,紫外检测器检测,外标法定量。结果表明:在所选择的色谱条件下,空白载体中肉桂醛、丁香酚、香芹酚和百里香酚能达到很好的分离;丁香酚、香芹酚和百里香酚在1.0~500 mg/L内线性关系良好,肉桂醛在0.10~50.0 mg/L内线性关系良好;丁香酚、香芹酚和百里香酚最低定量限为50 mg/kg,肉桂醛最低定量限为5.0 mg/kg;加标回收率为83.7%~112%,变异系数为0.70%~6.39%。该方法样品前处理简单、简便可靠,可用于混合型饲料添加剂中肉桂醛、丁香酚、香芹酚和百里香酚的同步检测。
The study established a liquid phase chromatographic method for simultaneous determination of cinnamaldehyde,eugenol,carvacrol and thymol in mixed feed additives.Feed samples were firstly extracted by methanol,after being isolated by C18 column(150 mm×4.6 mm×5μm),eventually detected by ultraviolet detector,and were quantified by external standard method.The results showed that under the chosen chromatographic conditions,cinnamaldehyde,eugenol,carvacrol and thymol were separated successfully;eugenol,carvacrol and thymol were linear in the range of 1.0 to 500 mg/L,cinnamaldehyde was linear in the range of 0.10 to 50.0 mg/L.The low quantitative limit of eugenol,carvacrol and thymol were 50 mg/kg,and the low quantitative limit of cinnamaldehyde was 5.0 mg/kg.The relative standard deviation(RSD)was 0.70%~6.39%when the recovery rate was 83.7%~112%.The method was proved to be simple,rapid and resproducible for determination of cinnamaldehyde,eugenol,carvacrol and thymol in mixed feed additives.
作者
张玮
李会荣
宫玲玲
ZHANG Wei;LI Hui-rong;GONG Ling-ling
出处
《饲料研究》
CAS
北大核心
2020年第2期64-69,共6页
Feed Research
关键词
混合型饲料添加剂
肉桂醛
丁香酚
香芹酚
百里香酚
液相色谱法
mixed feed additives
cinnamaldehyde
eugenol
carvacrol
thymol
liquid phase chromatographic method
