摘要
南极磷虾油中卵磷脂(lecithin,PC)型二十碳五烯酸(ecosapentaenoic acid,EPA)相比于非PC型EPA具有更高的生物活性和生物利用度。为鉴定南极磷虾油中PC型EPA的分布比例,通过薄层色谱法分离南极磷虾油中的PC,利用气相色谱方法对其中的EPA进行定量。测定6种不同来源南极磷虾油中PC型EPA的含量,利用南极磷虾油PC型EPA含量占总EPA含量的比例这一参数评价南极磷虾油的品质。研究结果表明,二十碳五烯酸甲酯(methyl ecosapentaenoic acid,EPA-ME)相比于内标十七烷酸甲酯(methyl heptadecanoate,C17:0-ME)的相对质量校正因子为1.52;所筛选出的优质南极磷虾油的PC型EPA含量占总EPA含量的比例在60%以上,最高可达70%。
Lecithin(PC)associated ecosapentaenoic acid(EPA)from Antarctic krill has higher biological activity and bioavailability than non-PC associated EPA.To quantify the distribution ratio of PC-associated EPA in krill oil,the PC in Antarctic krill oil was isolated by thin-layer chromatography and its EPA was quantified by gas chromatography.The content of PC-associated EPA in six kinds of Antarctic krill oil from different sources was accurately determined.Antarctic krill oil quality was evaluated using the ratio of PC-associated EPA to total EPA.The relative mass correction factor for methyl ecosapentaenoic acid(EPA-ME)was 1.52,compared with the internal standard,methyl heptadecanoate(C17:0-ME).The ratio of PC-associated EPA to total EPA in high quality Antarctic krill oil ranged from>60%to 70%.
作者
范雁
杨淼
姜君鹏
冯迪娜
薛松
FAN Yan;YANG Miao;JIANG Jun-peng;FENG Di-na;XUE Song(Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,Liaoning,China;University of Chinese Academy of Sciences,Beijing 100049,China;School of Bioengineering,Dalian University of Technology,Dalian 116024,Liaoning,China)
出处
《食品研究与开发》
CAS
北大核心
2021年第15期158-162,共5页
Food Research and Development
基金
吉林省科技发展计划项目(20200708050YY)
中国博士后科学基金资助项目(2020M670806)
关键词
南极磷虾油
卵磷脂
磷脂酰胆碱
二十碳五烯酸
品质分析
Antarctic krill oil
phospholipids
phosphatidylcholine
eicosapentaenoic acid
quality evaluation
